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Recombinant Human RNase H1/RNH1 protein (ab153634)

Reviews (1) Submit a question References (4)
SDS-PAGE - Recombinant Human RNase H1/RNH1 protein (ab153634)

    Key features and details

    • Expression system: Wheat germ
    • Purity: >= 80% Purified via GST Tag
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human RNase H1/RNH1 protein

    • 精製度

      >= 80 % Purified via GST Tag.
      Glutathione Sepharose 4 Fast Flow

    • 発現系

      Wheat germ

    • アクセッション番号

      O60930

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQV DRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLR EPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSS NGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQ NINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERL TQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

      • 予測される分子量

        59 kDa including tags

      • 領域

        1 to 286

      • タグ

        GST tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab153634 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid

    • 備考

      Protein concentration is above or equal to 0.03 mg/ml.

       This product was previously labelled as RNase H1

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • H1RNA
      • MGC108918
      • Ribonuclease H type II
      • RibonucleaseH1
      • RNaseH1
      • RNH1
      see all

    • 関連性

      Rnase H1 specifically degrades the RNA of RNA-DNA hybrids.

    • 細胞内局在

      Cytoplasmic

    画像

    • SDS-PAGE - Recombinant Human RNase H1/RNH1 protein (ab153634)
      SDS-PAGE - Recombinant Human RNase H1/RNH1 protein (ab153634)
      ab153634 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab153634 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab153634 は 4 報の論文で使用されています。

    • Nguyen K  et al. Chemical evolution of an autonomous DNAzyme with allele-specific gene silencing activity. Nat Commun 14:2413 (2023). PubMed: 37105964
    • Scopa C  et al. JUN upregulation drives aberrant transposable element mobilization, associated innate immune response, and impaired neurogenesis in Alzheimer's disease. Nat Commun 14:8021 (2023). PubMed: 38049398
    • Shiraishi T  et al. Synthesis and properties of oligodiaminogalactoses that bind to A-type oligonucleotide duplexes. Org Biomol Chem 20:8243-8258 (2022). PubMed: 36222332
    • Shiromoto Y  et al. ADAR1 RNA editing enzyme regulates R-loop formation and genome stability at telomeres in cancer cells. Nat Commun 12:1654 (2021). PubMed: 33712600

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Enzymatic activity of Human RNase H1 full length protein (ab153634)

     Excellent
    Abreviews
    abreview image
    Application
    Functional Studies
    We tested enzymatic activity of Human RNase H1 full length protein (ab153634). RNase H1 is an endonuclease that cleaves RNA in DNA-RNA duplex. To see the protein's cleavage activity, we used 363 nt 5'-32P labeled RNA that contains target sequence of our antisense oligonucleotide. We observed expected sized RNA fragments after 5 min incubation of the RNA-oligonucleotide duplex with the RNase H1 protein at 37°C (Figure A). We also compare the reaction with E.coli RNase H. The result shows that the human RNase H1 gives the same sized fragment as E. coli RNase H does (Figure B).
    Method:
    RNase H mediated cleavage of 5’-32P-RNA (363 nt). (A) 5’-32P-RNA (0.5 pmol) was mixed with 20 nt antisense oligonucleotides (3 pmol) in buffer containing 60 mM Tris-HCl (pH7.8), 60 mM KCl, 2.5 mM MgCl2, and 2 mM DTT. The mixtures were heated at 95 °C for 3 min and slowly cooled to room temperature. Subsequently, various amount of human RNase H1 was added to the mixtures and incubated at 37 °C for 5 min. the reactions were quenched by adding an equal amount of loading dye containing deionized formamide. The samples were heated at 95 °C for 5 min, then quickly cooled down on ice and loaded onto 5% polyacrylamide gel containing 7 M urea. (B) 5’-32P-RNA (0.5 pmol) was mixed with 20 nt antisense oligonucleotides (3 pmol) in buffer containing 60 mM Tris-HCl (pH7.8), 60 mM KCl, 2.5 mM MgCl2, and 2 mM DTT. The mixtures were heated at 95 °C for 3 min and slowly cooled to room temperature. Subsequently, 2.5 units E. coli RNase H was added to the mixtures and incubated at 37 °C. the reactions were quenched at 2, 5, 10, 20 and 30 min by adding an equal amount of loading dye containing deionized formamide. The samples were heated at 95 °C for 5 min, then quickly cooled down on ice and loaded onto 5% polyacrylamide gel containing 7 M urea.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Dec 29 2015

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