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Recombinant Human RGS10 protein (ab109947)

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SDS-PAGE - Recombinant Human RGS10 protein (ab109947)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    製品の詳細

    • 製品名

      Recombinant Human RGS10 protein

    • 精製度

      > 90 % SDS-PAGE.
      ab109947 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

    • 発現系

      Escherichia coli

    • アクセッション番号

      O43665

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGSSHHHHHH SSGLVPRGSH MGSH MFNRAVSRLSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPE GVKRFREFLKKEFSEENVLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSS KASSQVNVEGQSRLNEKILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSD LFLKHKRTEEEEEDLPDAQTAAKRASRIYNT

      • 予測される分子量

        24 kDa including tags

      • 領域

        1 to 181

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab109947 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Mass Spectrometry

    • 質量分析

      MALDI-TOF

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.058% Sodium chloride

    関連情報

    • 別名

      • Regulator of G protein signaling 10
      • Regulator of G-protein signaling 10
      • RGS10
      • RGS10_HUMAN

    • 機能

      Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

    • 配列類似性

      Contains 1 RGS domain.

    • 翻訳後修飾

      Isoform 3 is phosphorylated on Ser-16.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human RGS10 protein (ab109947)
      SDS-PAGE - Recombinant Human RGS10 protein (ab109947)
      ab109947 at 3 ug on an SDS-PAGE gel (15%)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab109947 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109947 は論文での使用が確認できていません。

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