AB63808

Recombinant human Rad51 protein (Active)

Name: Recombinant human Rad51 protein (Active) (ab63808) | Abcam
Brand: Abcam Limited
SKU: AB63808
Price: 116000 JPY
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(7 Publications)

Recombinant human Rad51 protein (Active) is a Human Full Length protein, in the 1 to 339 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

RAD51A, RECA, RAD51, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A

1 Images

SDS-PAGE - Recombinant human Rad51 protein (Active) (AB63808)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Rad51 protein (Active) (AB63808)

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学的活性

Retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.

アクセッション番号

Q06609

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 6 - 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0146% EDTA

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab63808 can be used as a WB positive control in conjunction with ab46981." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Studies on homologous recombination in mammals including human. ab63808 is also confirmed to possess single strand DNA stimulated ATPase activity." } } }

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製品の詳細

This protein can be used for:

1) Studies on homologous recombination mechanism and SOS response.

2) Useful in the screening using probe from library by promotion of DNA hybridization.

3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

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配列情報

[{"sequence":"GSHMAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD","proteinLength":"Full Length","predictedMolecularWeight":"37 kDa","actualMolecularWeight":null,"aminoAcidEnd":339,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q06609","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.

Biological function summary

Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.

Pathways

RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.

RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.

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製品の性状

製品の状態

Liquid

補足情報

Highly purified by several steps of chromatography.

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一般的な情報

機能

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 22325354, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 32640219). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed : 12205100, PubMed : 18417535, PubMed : 15226506, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (PubMed : 12205100, PubMed : 18417535, PubMed : 20231364, PubMed : 20348101, PubMed : 23509288, PubMed : 23754376, PubMed : 26681308, PubMed : 28575658, PubMed : 38459011). Recruited to resolve stalled replication forks during replication stress (PubMed : 27797818, PubMed : 31844045). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR (PubMed : 12442171, PubMed : 24141787). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (PubMed : 20413593). Also involved in interstrand cross-link repair (PubMed : 26253028).

配列の類似性

Belongs to the RecA family. RAD51 subfamily.

翻訳後修飾

Ubiquitinated by the SCF(FBH1) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Ubiquitinated by RFWD3 in response to DNA damage: ubiquitination leads to degradation by the proteasome, promoting homologous recombination (PubMed:28575658).. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination (PubMed:15665856). Phosphorylated at Ser-14 by PLK1, triggering phosphorylation at Thr-13 by CK2, thereby promoting interaction with TOPBP1 and recruitment to DNA damage sites during S-phase (PubMed:22325354, PubMed:26811421). Phosphorylation by ABL1 inhibits function (PubMed:9461559).

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information RAD51

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2631:253-266 PubMed36995671

2023

CRISPR/Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Selika Garza,Raehum Paik

Molecular cell 82:3985-4000.e4 PubMed36265486

2022

TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch.

Applications

Unspecified application

Species

Unspecified reactive species

Tribhuwan Yadav,Jia-Min Zhang,Jian Ouyang,Wendy Leung,Antoine Simoneau,Lee Zou

Bioorganic & medicinal chemistry 70:116923 PubMed35841829

2022

Comparison of ATP-binding pockets and discovery of homologous recombination inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Blay,Saule Gailiunaite,Chih-Ying Lee,Hao-Yen Chang,Ted Hupp,Douglas R Houston,Peter Chi

Neuron 109:2590-2603.e13 PubMed34197733

2021

Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Dheeraj S Roy,Ying Zhang,Tomomi Aida,Soonwook Choi,Qian Chen,Yuanyuan Hou,Nicholas E Lea,Keith M Skaggs,Juliana C Quay,Min Liew,Hannah Maisano,Vinh Le,Carter Jones,Jie Xu,Dong Kong,Heather A Sullivan,Arpiar Saunders,Steven A McCarroll,Ian R Wickersham,Guoping Feng

Cell 184:3267-3280.e18 PubMed34043941

2021

Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan J Wilde,Tomomi Aida,Ricardo C H Del Rosario,Tobias Kaiser,Peimin Qi,Martin Wienisch,Qiangge Zhang,Steven Colvin,Guoping Feng

Nature 594:283-288 PubMed33981036

2021

RNA transcripts stimulate homologous recombination by forming DR-loops.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Ouyang,Tribhuwan Yadav,Jia-Min Zhang,Haibo Yang,Esther Rheinbay,Hongshan Guo,Daniel A Haber,Li Lan,Lee Zou

Nature 571:521-527 PubMed31270457

2019

Isomerization of BRCA1-BARD1 promotes replication fork protection.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Daza-Martin,Katarzyna Starowicz,Mohammed Jamshad,Stephanie Tye,George E Ronson,Hannah L MacKay,Anoop Singh Chauhan,Alexandra K Walker,Helen R Stone,James F J Beesley,Jennifer L Coles,Alexander J Garvin,Grant S Stewart,Thomas J McCorvie,Xiaodong Zhang,Ruth M Densham,Joanna R Morris

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Recombinant human Rad51 protein (Active)