AB109943

Recombinant Human Rab3D protein (His tag N-Terminus)

Name: Recombinant Human Rab3D protein (ab109943) | Abcam
Brand: Abcam Limited
SKU: AB109943
Price: 88000 JPY
Availability: InStock

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Recombinant Human Rab3D protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 219 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

GOV, RAB16, RAB3D, Ras-related protein Rab-3D

1 Images

SDS-PAGE - Recombinant Human Rab3D protein (AB109943)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Rab3D protein (AB109943)

15% SDS-PAGE analysis of 3μg ab109943.

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

アクセッション番号

O95716

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.116% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC","proteinLength":"Full Length","predictedMolecularWeight":"26.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":219,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95716","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab3D also known as Ras-related protein Rab-3D belongs to the Rab family of small GTPases. It has a mass of approximately 25 kDa. Rab3D functions as a molecular switch that cycles between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking processes. This protein is highly expressed in secretory tissues such as exocrine pancreas salivary glands and osteoclasts where it plays a role in the secretory pathway.

Biological function summary

Rab3D plays an essential role in vesicle exocytosis modulating the secretion of enzymes hormones and other secretory products. Much of Rab3D's function depends on its interaction with other vesicle-associated proteins including Rab3-interacting molecules (RIMs) which are part of the secretory complex. Rab3D ensures proper docking and fusion of secretory vesicles with the plasma membrane.

Pathways

The Rab3D protein influences the exocytosis and endocytic pathways. It closely interacts with other members of the Rab family especially Rab27 and Rab8 which orchestrate various sub-tasks in vesicle trafficking. These pathways are vital for vesicle-mediated transport and require the regulated activity of Rab3D for proper cellular communication and secretion.

Rab3D links to bone resorption disorders specifically related to osteoclast function and bone density diseases like osteoporosis. Dysregulation in Rab3D activity can influence osteoclast function where Rab3D works alongside proteins such as Rab27a contributing to the excessive bone resorption noted in osteoporosis. Additionally altered Rab3D function may have implications in disorders related to exocrine gland secretion impacting conditions like Sjögren's syndrome.

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製品の性状

製品の状態

Liquid

補足情報

ab109943 was purified using conventional chromatography techniques.

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一般的な情報

機能

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB3D may be involved in the insulin-induced exocytosis of GLUT4-containing vesicles in adipocytes (By similarity).

配列の類似性

Belongs to the small GTPase superfamily. Rab family.

翻訳後修飾

Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and CHML.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information RAB3D

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Abcam product promise

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Recombinant Human Rab3D protein (His tag N-Terminus)