AB276411

Recombinant Human PVRL1/NECTIN1 protein (His tag)

Name: Recombinant Human PVRL1/NECTIN1 protein (His tag) (ab276411) | Abcam
Brand: Abcam Limited
SKU: AB276411
Price: 63000 JPY
Availability: InStock

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Recombinant Human PVRL1/NECTIN1 protein (His tag) is a Human Fragment protein, in the 1 to 334 aa range, expressed in HEK 293 cells, with >98%, < 1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

別名を表示する

CD111, HVEC, PRR1, PVRL1, NECTIN1, Nectin-1, Herpes virus entry mediator C, Herpesvirus Ig-like receptor, Nectin cell adhesion molecule 1, Poliovirus receptor-related protein 1, Herpesvirus entry mediator C, HveC, HIgR

1 Images

SDS-PAGE - Recombinant Human PVRL1/NECTIN1 protein (His tag) (AB276411)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PVRL1/NECTIN1 protein (His tag) (AB276411)

SDS-PAGE analysis of ab276411

Key facts

精製度

>98% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

HEK 293 cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

1. Measured by its binding ability in a functional ELISA. Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml. 2. Immobilized Recombinant Human Nectin 3 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag) (ab276411), the EC50 is 250-750 ng/mL.

アクセッション番号

Q15223

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"MARMGLAGAAGRWWGLALGLTAFFLPGVHSQVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAKKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNIT","proteinLength":"Fragment","predictedMolecularWeight":"35.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q15223","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein PVRL1 also known as NECTIN1 or nectin-1 functions mechanically as an immunoglobulin-like cell adhesion molecule. It plays a role in creating adherens junctions between cells operating as both a homophilic and heterophilic binding partner. Nectin-1 has a molecular mass of approximately 97 kDa. It is expressed prominently in epithelial tissues and particularly in the nervous system which suggests it plays an important role in cell-cell communication in these areas.

Biological function summary

Nectin-1 facilitates cellular processes through its involvement in cell adhesion complexes. It functions as part of a larger complex including proteins like afadin which links to the actin cytoskeleton and promotes cell junction integrity. This organization impacts cellular adhesion migration and proliferation. Nectin-1's adhesive functions are fundamental in maintaining structural tissues and transmitting signals within cells a necessity for development and tissue maintenance.

Pathways

Nectin-1 contributes to the activation of the MAPK/ERK signaling pathway central to regulating various cell functions including proliferation and differentiation. It also participates in the cadherin-catenin complex pathway working in conjunction with E-cadherin a critical protein in maintaining epithelial cell layer integrity. These pathways highlight nectin-1’s role as a signal transducer affecting how cells interact and react to their environment.

Nectin-1 is implicated in cleft lip and palate disorder. Mutations affecting nectin-1 can disrupt cell adhesion processes necessary for proper development of facial structures. Furthermore alterations in nectin-1 gene expression are associated with susceptibility to herpes simplex virus infections as nectin-1 acts as a viral entry receptor. In these conditions its interaction with closely related membrane proteins such as nectin-3 is significant in disease manifestation and progression.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system (PubMed : 21325282). Acts by forming homophilic or heterophilic trans-dimers (PubMed : 21325282). Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4 (By similarity). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Involved in synaptogegesis (By similarity). Has some neurite outgrowth-promoting activity (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN3 : NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Required for enamel mineralization (By similarity).. (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV (PubMed : 21980294, PubMed : 25231300, PubMed : 28381567, PubMed : 28542478, PubMed : 34587223, PubMed : 38857290, PubMed : 39048823, PubMed : 39048830, PubMed : 7721102, PubMed : 9616127, PubMed : 9657005). Constitutes the major receptor for herpes simplex virus 1/HHV-1 entry into host cells (PubMed : 34587223).

配列の類似性

Belongs to the nectin family.

翻訳後修飾

(Microbial infection) Ubiquitinated by CBL following infection by herpes simplex virus 1/HHV-1 and association with HHV-1 envelope glycoprotein D, leading to its removal from cell surface.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information NECTIN1

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Recombinant Human PVRL1/NECTIN1 protein (His tag)