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AB198147

Recombinant human Protein C (His tag C-Terminus)

Recombinant human Protein C (His tag C-Terminus)

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Recombinant human Protein C (His tag C-Terminus) is a Human Full Length protein, in the 1 to 461 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, PROC

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Functional Studies - Recombinant human Protein C (His tag C-Terminus) (AB198147)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Protein C (His tag C-Terminus) (AB198147)

Specific activity of ab198147.

SDS-PAGE - Recombinant human Protein C (His tag C-Terminus) (AB198147)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Protein C (His tag C-Terminus) (AB198147)

4-20% SDS-PAGE analysis of ab198147 (3 μg) with Coomassie staining.

Note : Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.

Key facts

精製度

>90% SDS-PAGE

発現系

HEK 293 cells

タグ

His tag C-Terminus

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific Activity: 90 pmol/min/μg.

Assay Conditions: 50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl2, 0.01% Brij-35, 100 μM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.

アクセッション番号

P04070

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Preservative: 1.7% Imidazole Constituents: 0.8% Sodium chloride, 0.79% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MWQLTSLLLFVATWGISGTPAPLDSVFSSSERAHQVLRIRKRANSFLEELRHSSLERECIEEICDFEEAKEIFQNVDDTLAFWSKHVDGDQCLVLPLEHPCASLCCGHGTCIDGIGSFSCDCRSGWEGRFCQREVSFLNCSLDNGGCTHYCLEEVGWRRCSCAPGYKLGDDLLQCHPAVKFPCGRPWKRMEKKRSHLKRDTEDQEDQVDPRLIDGKMTRRGDSPWQVVLLDSKKKLACGAVLIHPSWVLTAAHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP","proteinLength":"Full Length","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":461,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P04070","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein C is a vitamin K-dependent serine protease enzyme that plays an important role in blood coagulation. It is also known as autoprothrombin IIA or blood coagulation factor XIV. The molecular weight of Protein C is approximately 62 kDa. It is synthesized in the liver and circulates in the plasma as an inactive zymogen. The protein becomes activated on the surface of endothelial cells and platelets where it plays an anticoagulant role.
Biological function summary

Protein C serves as an important regulator in the coagulation pathway. Upon activation it joins with a cofactor known as Protein S to form the activated Protein C complex which inactivates clotting factors Va and VIIIa. This inactivation leads to a reduction in thrombin formation thereby preventing excessive blood clotting. The activated C complex works efficiently on phospholipid surfaces such as those found on cellular membranes.

Pathways

Protein C activation is an important step in the anticoagulant pathway and works to balance the procoagulant actions of other factors. Protein C functions in tandem with proteins such as thrombin which transforms it into its active form when bound to thrombomodulin on the endothelial cell surface. The activated Protein C pathway ensures proper blood flow by preventing unchecked clot propagation and is intricately tied with pathways involving thrombin and factors Va and VIIIa.

Protein C deficiency is associated with an increased risk of developing thromboembolic disorders like deep vein thrombosis and pulmonary embolism. Deficiency can be congenital or acquired resulting in uncontrolled coagulation and increased susceptibility to clot formation. Additionally impaired activity of protein C is related to a disorder known as purpura fulminans which is characterized by blood clot formation and skin necrosis. The relation of Protein C to these conditions often involves dysfunctional interactions with factors Va VIIIa and thrombin.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed : 25618265, PubMed : 39880037). Exerts a protective effect on the endothelial cell barrier function (PubMed : 25651845).

配列の類似性

Belongs to the peptidase S1 family.

翻訳後修飾

Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium.. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.

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製品プロトコール

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ターゲットの情報

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed : 25618265, PubMed : 39880037). Exerts a protective effect on the endothelial cell barrier function (PubMed : 25651845).
See full target information PROC
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