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Recombinant Human Proteasome 20S LMP2 protein (ab105592)

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SDS-PAGE - Recombinant Human Proteasome 20S LMP2 protein (ab105592)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    製品の詳細

    • 製品名

      Recombinant Human Proteasome 20S LMP2 protein

    • 精製度

      > 90 % SDS-PAGE.
      ab105592 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

    • 発現系

      Escherichia coli

    • アクセッション番号

      P28065

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGSSHHHHHHSSGLVPRGSHMTTIMAVEFDGGVVMGSDSRVSA GEAVV NRVFDKLSPLHERIYCALSGSAADAQAVADMAAYQLELHGIELEE PPL VLAAANVVRNISYKYREDLSAHLMVAGWDQREGGQVYGTLGGMLTRQ P FAIGGSGSTFIYGYVDAAYKPGMSPEECRRFTTDAIALAMSRDGSSGGV IYLVTITAAGVDHRVILGNELPKFYDE

      • 予測される分子量

        24 kDa including tags

      • 領域

        21 to 219

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab105592 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Mass Spectrometry

    • 質量分析

      MALDI-TOF

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    関連情報

    • 別名

      • Beta1i
      • Large multifunctional peptidase 2
      • Large multifunctional protease 2
      • LMP 2
      • LMP2
      • Low molecular mass protein 2
      • Macropain chain 7
      • MGC70470
      • Multicatalytic endopeptidase complex chain 7
      • OTTHUMP00000062982
      • Proteasome (prosome macropain) subunit beta type 9
      • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
      • Proteasome beta 9 subunit
      • Proteasome catalytic subunit 1i
      • Proteasome chain 7
      • Proteasome related gene 2
      • Proteasome subunit beta 6i
      • Proteasome subunit beta type 9
      • Proteasome subunit beta type-9
      • Proteasome subunit beta-1i
      • PSB9_HUMAN
      • PSMB 9
      • PSMB6i
      • PSMB9
      • Really interesting new gene 12 protein
      • RING 12
      • RING12
      • RING12 protein
      see all

    • 機能

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

    • 配列類似性

      Belongs to the peptidase T1B family.

    • 発生段階

      Highly expressed in immature dendritic cells (at protein level).

    • 翻訳後修飾

      Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

    • 細胞内局在

      Cytoplasm. Nucleus.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Proteasome 20S LMP2 protein (ab105592)
      SDS-PAGE - Recombinant Human Proteasome 20S LMP2 protein (ab105592)

      SDS-PAGE analysis of 3µg ab105592 under reducing condition and visualized by coomassie blue stain.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab105592 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab105592 は論文での使用が確認できていません。

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