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AB80376

Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus)

Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus)

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(2 Publications)

Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus) is a Human Full Length protein, in the 2 to 710 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

PEP, PREP, Prolyl endopeptidase, PE, Post-proline cleaving enzyme

2 Images
Functional Studies - Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus) (AB80376)
  • FuncS

Unknown

Functional Studies - Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus) (AB80376)
SDS-PAGE - Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus) (AB80376)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Prolyl Endopeptidase protein (His tag N-Terminus) (AB80376)

Lane 1 : ab80376 on 10% SDS-PAGE, Coomassie staining, 4μg.
Lane 2 : Protein marker.

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Specific Activity: ≥ 12 pmol/min/μg. Assay condition: 50 mM Tris (pH 7.5), 100 μg BSA, 20 μM substrate and 500 ng Prolyl Endopeptidase protein at room temperature for 30 min.

アクセッション番号

P48147

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Proteins & Peptides

AB254309

Recombinant human Alpha-Synuclein protein filament

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament (AB254309)

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Proteins & Peptides

AB202613

Recombinant Human LAP3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human LAP3 protein (His tag N-Terminus) (AB202613)

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Proteins & Peptides

AB133147

Recombinant Human FNDC5 protein

SDS-PAGE - Recombinant Human FNDC5 protein (AB133147)

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Proteins & Peptides

AB86706

Recombinant Human Ataxin 3 protein (Tag Free)

SDS-PAGE - Recombinant Human Ataxin 3 protein (Tag Free) (AB86706)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MHHHHHHLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP","proteinLength":"Full Length","predictedMolecularWeight":"81.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":710,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P48147","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prolyl endopeptidase also known as prolyl oligopeptidase is a serine peptidase with a molecular mass of about 80 kDa. It specifically cleaves peptide bonds at the C-terminal side of proline residues within short peptides. Prolyl endopeptidase is expressed widely including in brain and peripheral tissues making it a significant target in various cellular processes. This enzyme features a catalytic triad typical of serine proteases which consists of serine histidine and aspartate residues.
Biological function summary

Prolyl endopeptidase participates in the modulation and degradation of proline-containing peptides impacting neuropeptide activity and peptide hormone regulation. It operates largely outside of any complex functioning independently in its enzymatic roles. Its activity influences processes such as cell signaling and neurotransmission by altering the lifespans of bioactive peptides contributing to cellular homeostasis and communication efficacy.

Pathways

Four enzymes interact in a cascade that includes prolyl endopeptidase within the renin-angiotensin system and other neuropeptide pathways. Particularly noteworthy is its involvement in the hydrolysis of small bioactive peptides which links it to proteins like angiotensin-converting enzyme. Prolyl endopeptidase manipulates peptide availability influencing blood pressure control and cognitive functions by its participation in peptide-related pathways.

Four enzymes are implicated in cognitive disorders and cardiovascular diseases with prolyl endopeptidase playing a considerable role. Its aberrant activity relates to neurodegenerative diseases such as Alzheimer's disease and conditions like hypertension. In Alzheimer's altered activity affects amyloid-beta peptide processing and interaction with tau protein while in hypertension its regulation or dysregulation impacts peptide substrates affiliated with blood pressure modulation.
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一般的な情報

機能

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

配列の類似性

Belongs to the peptidase S9A family.

翻訳後修飾

The N-terminus is blocked.

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製品プロトコール

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ターゲットの情報

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
See full target information PREP
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Israel journal of chemistry 63: PubMed37982048

2023

DPP8/9 are not Required to Cleave Most Proline-Containing Peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Abir Bhattacharjee,Daniel A Bachovchin

Molecular cell 73:446-457.e6 PubMed30612880

2019

Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection.

Applications

Unspecified application

Species

Unspecified reactive species

Abul Arif,Jie Jia,Belinda Willard,Xiaoxia Li,Paul L Fox
View all publications
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