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Recombinant human PRMT3 protein (ab167953)

Submit a review Submit a question References (1)
Functional Studies - Recombinant human PRMT3 protein (ab167953)
  • SDS-PAGE - Recombinant human PRMT3 protein (ab167953)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, WB, Functional Studies

製品の詳細

  • 製品名

    Recombinant human PRMT3 protein

  • 生理活性

    The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.

  • 精製度

    > 90 % Densitometry.
    Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    Q8WUV3

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MCSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPC LFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPN GLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQN PHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLN KLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAK NKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAV IPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMC TAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEA LKGKVTVHKSKKDPRSLTVTLTLNNSTQTYGLQ

    • 予測される分子量

      97 kDa including tags

    • 領域

      1 to 531

    • タグ

      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab167953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

    Functional Studies

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • 2010005E20Rik
    • 2410018A17Rik
    • AL033309
    • ANM3_HUMAN
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 3
    • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
    • HMT1 hnRNP methyltransferase like 3
    • HMT1 hnRNP methyltransferase like 3 S. cerevisiae
    • HMT1-like 3
    • hnRNP methyltransferase like 3
    • HRMT1L3
    • PRMT 3
    • PRMT3
    • Protein arginine methyltransferase 3
    • Protein arginine N methyltransferase 3
    • Protein arginine N-methyltransferase 3
    see all

  • 機能

    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.

  • 配列類似性

    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 C2H2-type zinc finger.

  • ドメイン

    The zinc-finger is responsible for substrate specificity.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

画像

  • Functional Studies - Recombinant human PRMT3 protein (ab167953)
    Functional Studies - Recombinant human PRMT3 protein (ab167953)
    The specific activity of ab167953 was determined to be 12 nmol/min/mg as per activity assay protocol.
  • SDS-PAGE - Recombinant human PRMT3 protein (ab167953)
    SDS-PAGE - Recombinant human PRMT3 protein (ab167953)
    SDS-PAGE analysis of ab167953.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab167953 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab167953 は 1 報の論文で使用されています。

  • Hsu MC  et al. Protein Arginine Methyltransferase 3 Enhances Chemoresistance in Pancreatic Cancer by Methylating hnRNPA1 to Increase ABCG2 Expression. Cancers (Basel) 11:N/A (2018). PubMed: 30577570

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