Recombinant Human PRL-1 protein (ab42567)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: Inhibition Assay
製品の詳細
-
製品名
Recombinant Human PRL-1 protein
PRL-1 タンパク質・ペプチド 製品一覧 -
生理活性
0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30?C. Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. -
精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
20 kDa including tags -
領域
2 to 173 -
タグ
His tag C-Terminus
-
特性
Our Abpromise guarantee covers the use of ab42567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Inhibition Assay
-
製品の状態
Liquid -
備考
0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30?C. Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 0.0584% EDTA, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride
関連情報
-
別名
- HH 72
- HH72
- Hypothetical protein DKFZp779M0721
see all -
機能
Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. -
組織特異性
Expressed in bone marrow, lymph nodes, T lymphocytes, spleen, thymus and tonsil. Overexpressed in tumor cell lines. -
配列類似性
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain. -
発生段階
Expressed in fetal liver. -
翻訳後修飾
Farnesylated. Farnesylation is required for membrane targeting. Unfarnesylated forms are shifted into the nucleus. -
細胞内局在
Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm. Cytoplasm > cytoskeleton > spindle. And mitotic spindle. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab42567 は論文での使用が確認できていません。