AB268879

Recombinant human PPP2R1A + PP2A alpha protein (Active)

Name: Recombinant human PPP2R1A + PP2A alpha protein (Active) (ab268879) | Abcam
Brand: Abcam Limited
SKU: AB268879
Price: 75000 JPY
Availability: InStock

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Recombinant human PPP2R1A + PP2A alpha protein (Active) is a Human Full Length protein, in the 1 to 309 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.

別名を表示する

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PP2Aa, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A

2 Images

Functional Studies - Recombinant human PPP2R1A + PP2A alpha protein (Active) (AB268879)

FuncS

Supplier Data

Functional Studies - Recombinant human PPP2R1A + PP2A alpha protein (Active) (AB268879)

The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).

SDS-PAGE - Recombinant human PPP2R1A + PP2A alpha protein (Active) (AB268879)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PPP2R1A + PP2A alpha protein (Active) (AB268879)

SDS-PAGE analysis of ab268879.

Key facts

精製度

>75% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).

アクセッション番号

P30153

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 1.05% MOPS, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Proteins co-expressed.

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配列情報

[{"sequence":"MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":309,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P67775","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P30153","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R1A also referred to as PP2A alpha is a regulatory subunit of the protein phosphatase 2A (PP2A) complex. The protein has a mass of approximately 65 kDa. It functions mechanically as a scaffold subunit within the PP2A holoenzyme serving to coordinate the interaction between catalytic and regulatory subunits of the complex. This protein is widely expressed across various tissues which reflects its role in numerous cellular processes.

Biological function summary

The activity of PPP2R1A is essential for the dephosphorylation of serine/threonine residues on proteins allowing significant regulation of various cellular functions. The protein is an important part of the PP2A complex a heterotrimer that consists of structural (A) catalytic (C) and regulatory (B) subunits. This complex contributes to the control of cell growth and division by maintaining appropriate levels of phosphorylation within the cell impacting various signaling pathways.

Pathways

The functional activity of PPP2R1A integrates into critical pathways like the MAPK signaling pathway and cell cycle regulation. It modulates the activities of kinases and other proteins such as AKT and MEK which are critical to these pathways and are important in processes like cell proliferation and apoptosis. PPP2R1A's regulatory role ensures proper signal transduction within these pathways for maintaining cellular homeostasis.

The malfunction or mutation of PPP2R1A has been linked to cancers such as lung and colorectal cancer. Aberrant signaling within the pathways it regulates can lead to unregulated cell growth and contribute to tumorigenesis. Furthermore the protein's dysfunction can be associated with disorders connected to AKT signaling dysregulation reinforcing the importance of PPP2R1A's role in maintaining cellular structure and function.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit (PubMed : 15525651, PubMed : 16580887, PubMed : 33243860, PubMed : 33633399, PubMed : 34004147, PubMed : 8694763). Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed : 15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed : 16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 18782753, PubMed : 33633399). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling (PubMed : 18782753, PubMed : 33633399). Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753, PubMed : 33633399). Key mediator of a quality checkpoint during transcription elongation as part of the Integrator-PP2A (INTAC) complex (PubMed : 33243860, PubMed : 34004147). The INTAC complex drives premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : within the INTAC complex, acts as a scaffolding subunit for PPP2CA, which catalyzes dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, thereby preventing transcriptional elongation (PubMed : 33243860, PubMed : 34004147). Regulates the recruitment of the SKA complex to kinetochores (PubMed : 28982702).

配列の類似性

Belongs to the phosphatase 2A regulatory subunit A family.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

追加のターゲット

PPP2CA

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Recombinant human PPP2R1A + PP2A alpha protein (Active)