Recombinant human PPP2R1A + PP2A alpha protein (Active) (ab268879)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PPP2R1A + PP2A alpha protein (Active) -
生理活性
The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).
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精製度
> 75 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFL -
分子量情報
Approx 38kDa by SDS-PAGE -
領域
1 to 309 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_002715
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アミノ酸配列 2
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生物種
Human -
分子量情報
Approx 65 kDa by SDS-PAGE -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_014225
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab268879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Proteins co-expressed.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 1.05% MOPS, 1.74% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
PPP2R1A: Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1. PP2A alpha: Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
画像
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Functional Studies - Recombinant human PPP2R1A + PP2A alpha protein (ab268879)
The specific activity of ab268879 was ~ 4,000 nmol phosphate released/min/mg in an assay using a Thr-triphosphopeptide substrate (KRT(p)IRR).
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SDS-PAGE - Recombinant human PPP2R1A + PP2A alpha protein (ab268879)SDS-PAGE analysis of ab268879.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab268879 は論文での使用が確認できていません。