Recombinant human PPM1A protein (ab51205)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PPM1A protein
PPM1A タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: > 8,000units/mg of PPM1A. Unit Definition - one unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate. -
精製度
> 95 % SDS-PAGE.
ab51205 was purified by conventional column chromatography. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW -
領域
1 to 382 -
タグ
His tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab51205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.0308% DTT, 0.158% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.0126% Manganese chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Protein phosphatase 2C isoform alpha
- EC 3.1.3.16
- FLJ42306
see all -
機能
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. -
配列類似性
Belongs to the PP2C family. -
細胞内局在
Nucleus. - Information by UniProt
画像
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SDS-PAGE - Recombinant human PPM1A protein (ab51205)10% SDS-PAGE gel loaded with ab51205, PP2C alpha protein (His tag).
Predicted molecular weight 46.6 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab51205 は論文での使用が確認できていません。