AB101207

Recombinant human PPIL4 protein (His tag N-Terminus)

Name: Recombinant human PPIL4 protein (ab101207) | Abcam
Brand: Abcam Limited
SKU: AB101207
Price: 115000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Recombinant human PPIL4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 512 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec, FuncS.

別名を表示する

Peptidyl-prolyl cis-trans isomerase-like 4, PPIase, Cyclophilin-like protein PPIL4, Rotamase PPIL4, PPIL4

1 Images

SDS-PAGE - Recombinant human PPIL4 protein (His tag N-Terminus) (AB101207)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPIL4 protein (His tag N-Terminus) (AB101207)

ab101207 (3 μg) analysed by 15% SDS PAGE.
Molecular size on SDS-PAGE will appear higher than the predicted molecular weight of 59.4 kDa.

Key facts

精製度

>85% SDS-PAGE

ab101207 was purified using conventional chromatography and is >85 % pure by SDS-PAGE.

発現系

Escherichia coli

アプリケーション

SDS-PAGE, FuncS, Mass Spec

applications

生物活性

Yes

生物学的活性

Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 μmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.

アクセッション番号

Q8WUA2

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

style

left-column

style

left-column

配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAVLLETTLGDVVIDLYTEERPRACLNFLKLCKIKYYNYCLIHNVQRDFIIQTGDPTGTGRGGESIFGQLYGDQASFFEAEKVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDGVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLLIPDRSPEPTREQLDSGRIGADEEIDDFKGRSAEEVEEIKAEKEAKTQAILLEMVGDLPDADIKPPENVLFVCKLNPVTTDEDLEIIFSRFGPIRSCEVIRDWKTGESLCYAFIEFEKEEDCEKAFFKMDNVLIDDRRIHVDFSQSVAKVKWKGKGGKYTKSDFKEYEKEQDKPPNLVLKDKVKPKQDTKYDLILDEQAEDSKSSHSHTSKKHKKKTHHCSEEKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLYERERSKKRDRSRSPKKSKDKEKSKYR","proteinLength":"Full Length","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":512,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8WUA2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PPIL4 also known as Peptidylprolyl Isomerase Like 4 is a member of the cyclophilin family of proteins. It exhibits peptidyl-prolyl cis-trans isomerase activity facilitating the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The protein has a molecular weight of around 56 kDa and is ubiquitously expressed in various tissues throughout the body.

Biological function summary

PPIL4 participates in protein folding and assembly and it plays a role in maintaining cellular functions. This protein is part of complexes involved in RNA processing and mRNA splicing within the cell. It may interact with other spliceosomal components indicating its involvement in the regulation of gene expression through alternative splicing.

Pathways

PPIL4 is involved in the regulation of mRNA splicing influencing the expression of genes at the post-transcriptional level. It is connected to the spliceosome pathway where it associates with proteins like SNRNP200 and other spliceosomal proteins to modulate pre-mRNA splicing. This connection aids in the precise control of gene expression necessary for various cellular processes.

Altered PPIL4 function has implications in certain cancers and neurodegenerative diseases. Changes in PPIL4 expression may link to the disruption of normal splicing activities contributing to malignancies like adenocarcinomas. The protein's association with complex components like SNRNP200 could play a role in these diseases by affecting RNA processing stability and fidelity which are critical for maintaining normal cellular activities.

style

left-column

一般的な情報

機能

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity).

配列の類似性

Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.

細胞内局在性

Nucleus

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity).

See full target information PPIL4

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human PPIL4 protein (His tag N-Terminus)