Recombinant Human PPIH protein (ab184587)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, ELISA, WB
製品の詳細
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製品名
Recombinant Human PPIH protein
PPIH タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MKHHHHHHAS AVANSSPVNP VVFFDVSIGG QEVGRMKIEL FADVVPKTAE NFRQFCTGEF RKDGVPIGYK GSTFHRVIKD FMIQGGDFVN GDGTGVASIY RGPFADENFK LRHSAPGLLS MANSGPSTNG CQFFITCSKC DWLDGKHVVF GKIIDGLLVM RKIENVPTGP NNKPKLPVVI SQCGEM -
予測される分子量
20 kDa including tags -
領域
2 to 177 -
タグ
His tag N-Terminus -
配列の追加情報
The N terminal His tag is an extra 10 amino acids long.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab184587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
ELISA
Western blot
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質量分析
LC-MS/MS -
製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Filtered (0.4 µm) -
再構成Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml.
関連情報
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別名
- Cyclophilin H
- CYP-20
- CypH
see all -
機能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone. -
配列類似性
Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.
Contains 1 PPIase cyclophilin-type domain. -
細胞内局在
Nucleus speckle. Cytoplasm. Colocalizes with spliceosomal snRNPs. A small proportion may also be cytoplasmic. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human PPIH protein (ab184587)
14 % SDS-PAGE separation of Human PPIH
MW: Molecular weight marker
Lane 1: reduced and boiled sample, 2.5 μg
Lane 2: non-reduced and non-boiled sample, 2.5 μg
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab184587 は論文での使用が確認できていません。
