Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active)
Polymeric immunoglobulin receptor/PIGR タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA.
Immobilized ab174011 at 2 μg/ml, the concentration of Human IgM that produces 50% of the optimal binding response is appoximately 20-200 ng/ml. -
精製度
> 98 % SDS-PAGE.
ab174011 was lyophilized from 0.22 µm filtered solution. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
KSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLI SSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRG LSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQ IGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQY LCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAK FLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKE DAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGGSVA VLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEE PGNGTFTVILNQLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPG NVTAVLGETLKVPCHFPCKFSSYEKYWCKWNNTGCQALPSQDEGPSKAFV NCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAVYVAVEERKAA GSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRLFAEEKAVADTRDQA DGSRASVDSGSSEEQGGSSR -
予測される分子量
69 kDa including tags -
領域
19 to 638 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab174011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
ELISA
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製品の状態
Lyophilized -
備考
Previously labelled as PIGR.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is approximately about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- Hepatocellular carcinoma associated protein TB6
- Hepatocellular carcinoma-associated protein TB6
- MGC125361
see all -
機能
This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. -
配列類似性
Contains 5 Ig-like V-type (immunoglobulin-like) domains. -
翻訳後修飾
N-glycosylation is not necessary for Ig binding. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011)Human pIgR (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
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SDS-PAGE - Recombinant human Polymeric immunoglobulin receptor/PIGR protein (Active) (ab174011)The purity of ab174011 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue.
DTT-reduced Protein migrates as 85-95 kDa due to glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab174011 は論文での使用が確認できていません。
