Recombinant human PKC iota protein (ab60850)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant human PKC iota protein -
精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- aPKC lambda/iota
- aPKC-lambda/iota
- Atypical protein kinase C lambda/iota
see all -
機能
Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). -
組織特異性
Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメイン
The OPR domain mediates interaction with SQSTM1.
The C1 domain does not bind diacylglycerol (DAG). -
翻訳後修飾
On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation. -
細胞内局在
Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A. - Information by UniProt
画像
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Functional Studies - Recombinant human PKC iota protein (ab60850)The specific activity of PKC iota (ab60850) was determined to be 572 nmol/min/mg as per activity assay protocol and was equivalent to 564 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human PKC iota protein (ab60850)SDS PAGE analysis of ab60850 -
SDS-PAGE - Recombinant human PKC iota protein (ab60850)SDS PAGE analysis of ab60850 -
Functional Studies - Recombinant human PKC iota protein (ab60850)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC iota protein (ab60850)ab60850 on SDS-PAGE, MW ~98 kDa. -
Western blot - Recombinant human PKC iota protein (ab60850)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab60850 は論文での使用が確認できていません。