Recombinant human PKC epsilon protein (ab60847)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant human PKC epsilon protein
PKC epsilon タンパク質・ペプチド 製品一覧 -
精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab60847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 1.74% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- KPCE_HUMAN
- MGC125656
- MGC125657
see all -
機能
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻訳後修飾
Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. - Information by UniProt
画像
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Functional Studies - Recombinant human PKC epsilon protein (ab60847)The specific activity of PKC epsilon (ab60847) was determined to be 1122 nmol/min/mg as per activity assay protocol and was equivalent to 1398 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human PKC epsilon protein (ab60847)SDS PAGE analysis of ab60847 -
SDS-PAGE - Recombinant human PKC epsilon protein (ab60847)SDS PAGE analysis of ab60847 -
Functional Studies - Recombinant human PKC epsilon protein (ab60847)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC epsilon protein (ab60847)ab60847 on SDS-PAGE, MW ~110 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab60847 は論文での使用が確認できていません。