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Recombinant human PKA beta (catalytic subunit) protein (ab60837)

Submit a review Q&A (2)
Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

製品の詳細

  • 製品名

    Recombinant human PKA beta (catalytic subunit) protein
    PKA beta (catalytic subunit) タンパク質・ペプチド 製品一覧

  • 精製度

    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry. Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 予測される分子量

      65 kDa including tags

    • タグ

      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab60837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 製品の状態

    Liquid

  • 備考

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
    • cAMP-dependent protein kinase catalytic subunit beta
    • KAPCB_HUMAN
    • PKA C beta
    • PKA C-beta
    • PKACB
    • Prkacb
    • protein kinase A catalytic subunit beta
    • Protein kinase cAMP dependent catalytic beta
    see all

  • 機能

    Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

  • 組織特異性

    Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.

  • 細胞内局在

    Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt

画像

  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    The specific activity of PKA beta (catalytic subunit) (ab60837) was determined to be 430 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS PAGE analysis of ab60837
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS PAGE analysis of ab60837
  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    ab60837 on SDS-PAGE, MW ~65 kDa.
  • Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab60837 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab60837 は論文での使用が確認できていません。

レビューと Q&A

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Question

PKA beta (catalytic subunit) protein (Active) (ab60837)

I have not been able to get the enzyme to phosphorylate a known substrate that I have made, i have tried several conditions as theproduct information sheet doesn't specify the best assay conditions. I am not sure whether the problem is with the enzyme or the substrate - do you sell/can you recommend a substrate that I can use as a control for PKA?

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I contacted the source of this protein and they would liketo receivedetailed information with regards to your protocol, substrate information, lot number.
They need to have a thorough understanding of whathas beenperformed prior to make recommendations
This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the protein.

I look forward to receiving your reply.

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Question

Hi
I have recently purchased your an abcam product through sapphire biosciences


PKA beta (catalytic subunit) protein (Active) (ab60837)


I have not been able to get the enzyme to phosphorylate a known substrate that I have made, i have tried several conditions as theproduct information sheet doesn't specify the best assay conditions. I am not sure whether the problem is with the enzyme or the substrate - do you sell/can you recommend a substrate that I can use as a control for PKA?

Thanks
--

Read More
Answer

Thank you for your inquiry.

I can confirm that ab60837 was used in a kinase assay with CREBtide synthetic peptide substrate (KRREILSRRPSYR) , stock 1mg/ml.

This is available for purchase from other sources.

I attached the kinase assay protocol for your information.

Please let me know if the problems persist with the positive control.

We are happy to confirm that this product is tested and guaranteed for activity assays. In the event that a product is not functioning as stated on the datasheet, we happily offer technical help and a free of charge replacement or credit note.

https://www.abcam.com/index.html?pageconfig=resource&rid=10372&pid=10000&source=pagetrap&viapagetrap=abpromise

I wish you good luck with your research.

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