AB60837

Recombinant human PKA beta (catalytic subunit) protein

Name: Recombinant human PKA beta (catalytic subunit) protein (ab60837) | Abcam
Brand: Abcam Limited
SKU: AB60837
Price: 61000 JPY
Availability: InStock

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Recombinant human PKA beta (catalytic subunit) protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS, WB.

別名を表示する

cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB

5 Images

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

FuncS

Unknown

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

The specific activity of PKA beta (catalytic subunit) (ab60837) was determined to be 430 nmol/min/mg as per activity assay protocol

FuncS

Unknown

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

Sample Kinase Activity Plot.

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Western blot - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

All lanes:

Anti-PKA beta (catalytic subunit) antibody (ab94612) at 1 µg/mL

Lane 1:

Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.1 µg

Lane 2:

Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

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Exposure time: 1min

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

ab60837 on SDS-PAGE, MW ~65 kDa.

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

SDS PAGE analysis of ab60837

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

FuncS, WB, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

P22694

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab60837 can be used as a WB positive control in conjunction with ab94612." } } }

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製品の詳細

ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P22694","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PKA beta (catalytic subunit) also known as PRKACB or Protein Kinase A catalytic subunit beta plays a pivotal role in cellular signaling mechanisms. Mechanically it catalyzes the transfer of a phosphate group from ATP to a serine or threonine residue in target proteins impacting various cellular processes. This catalytic subunit has a molecular weight of approximately 40 kDa. It expresses widely in many tissues including the brain heart and skeletal muscle where it contributes to regulating metabolic and transcriptional activities.

Biological function summary

The PKA beta catalytic subunit functions in a larger context as part of the Protein Kinase A (PKA) complex which is a central player in the cyclic AMP (cAMP) signaling pathway. This complex acts as an effector of cAMP translating extracellular signals into intracellular actions by phosphorylating a wide range of target proteins. As a part of this complex it modulates activities related to glucose metabolism cell proliferation and gene expression reflecting its involvement in many cellular processes.

Pathways

PKA beta is integrally involved in the cAMP signaling and insulin signaling pathways. In the cAMP pathway it activates by binding to cAMP leading to dissociation from regulatory subunits and subsequent activation of substrate proteins. In the insulin signaling pathway it interacts with proteins like PI3K and Akt playing an important role in regulating glucose homeostasis and lipid metabolism. These pathways illustrate the PKA beta's importance in maintaining cellular and systemic metabolic balance.

The dysfunction of PKA beta is associated with conditions such as cardiac hypertrophy and certain types of cancer. Elevations or disruptions in PKA beta activity can lead to alterations in cell growth and metabolic regulation. PKA beta's relationship with proteins like CREB and Bcl-2 through these disorders has been observed where aberrant phosphorylation mediated by PKA can drive pathological cell survival proliferation and growth linking malfunction directly to disease progression.

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製品の性状

製品の状態

Liquid

補足情報

Purity: >90% as determined by densitometry. Affinity purified.

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一般的な情報

機能

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs (PubMed : 12420224, PubMed : 21423175, PubMed : 31112131). PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed : 12420224, PubMed : 21423175). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed : 12420224, PubMed : 21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed : 21880142). Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (PubMed : 31112131).

配列の類似性

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

翻訳後修飾

Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information PRKACB

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Recombinant human PKA beta (catalytic subunit) protein