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Recombinant Human PINK1 protein (denatured) (ab116177)

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SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human PINK1 protein (denatured)

    • 精製度

      > 90 % SDS-PAGE.
      ab116177 was purified using conventional chromatography.

    • 発現系

      Escherichia coli

    • アクセッション番号

      Q9BXM7

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL

      • 予測される分子量

        38 kDa

      • 領域

        156 to 507

    特性

    Our Abpromise guarantee covers the use of ab116177 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 6.01% Urea, 0.32% Tris HCl, 5% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • BRPK
      • FLJ27236
      • mitochondrial
      • PARK 6
      • PARK6
      • Phosphatase and Tensin Homolog
      • PINK 1
      • PINK1
      • PINK1_HUMAN
      • Protein kinase BRPK
      • PTEN induced putative kinase 1
      • PTEN induced putative kinase protein 1
      • PTEN-induced putative kinase protein 1
      • Serine/threonine kinase PINK1 mitochondrial
      • Serine/threonine protein kinase PINK1 mitochondrial
      • Serine/threonine-protein kinase PINK1
      see all

    • 機能

      Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.

    • 組織特異性

      Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

    • 関連疾患

      Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.

    • 配列類似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Autophosphorylated.

    • 細胞内局在

      Mitochondrion outer membrane. Cytoplasm > cytosol.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)
      SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)
      ab116177 on a 15% SDS-PAGE (3ug)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab116177 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab116177 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Hi,
    I have a few questions about your product ab116177 which is the recombinant protein PINK1.

    How was the protein purified?

    Does it have a tag?

    Why is 1M urea in the final buffer?

    How do you know it is autophosphoryated?

    Is it active?



    Many thanks,
    Ed



    E. Wheatley PhD

    Cancer Research Technology Ltd

    Wolfson Institute for Biomedical Research

    Gower Street

    London WC1E 6BT

    Tel: (+44) 020 76790927

    Fax:(+44) 020 7796 3907

    E-mail: ewheatley@cancertechnology.com




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    This communication and any attachments contain information which is confidential and may also be privileged. It is for the exclusive use of the intended recipient(s). If you are not the intended recipient(s) please note that any form of disclosure, distribution, copying or use of this communication or the information in it or in any attachments is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender and delete the email and destroy any copies of it.

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    Answer

    Thank you for your enquiry.

    I am pleased to help provide some answers:

    Q1. How was the protein purified? I am sorry to confirm that this particular product is sourced externally and we regrettably are not able to share manufacturing process information on this occasion.

    Q2. Does it have a tag? No, it does not have a tag.

    Q3. Why is 1 M urea in the final buffer? The urea helps prevent aggregation.

    Q4. Do you know if the protein is phosphorylated? If so, how has this been assessed? We have not confirmed if this protein is phosphorylated, but we suspect it isn’t. It’s most likely denatured.

    Q5. Is the protein active? I am sorry this has not been tested. It is unlikely to be active if it is denatured.

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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