Recombinant Human PGP9.5 protein (Tagged) (ab185598)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human PGP9.5 protein (Tagged)
PGP9.5 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
The final product was refolded using a unique “emperature shift inclusion body refolding technology and chromatographically purified. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALL LLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVA NNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEG QCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKV CREFTEREQGEVRFSAVALC -
予測される分子量
24 kDa -
領域
1 to 220 -
タグ
His-T7 tag N-Terminus -
配列の追加情報
Mature protein contructed with a T7-His-TEV cleavage site Tag (29 amino acids) fusion at the N-terminal.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab185598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
備考
1. May be used for in vitro UCHL1 protein mediated neuronal cell differentiation regulation study with “ProFectin” based intracellular delivery of this protein.
2. May be used for UCHL1 protein – protein interaction assay.
3. As Enzymatic substrate for various proteases.
4. Potential diagnostic biomarker protein for various cancer diseases.
5. May be used for specific antibody production.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and
Sucrose.
関連情報
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別名
- Epididymis luminal protein 117
- Epididymis secretory protein Li 53
- HEL 117
see all -
機能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
組織特異性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
関連疾患
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
配列類似性
Belongs to the peptidase C12 family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185598 は論文での使用が確認できていません。
