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AB101059

Recombinant Human PGLS protein (His tag N-Terminus)

Recombinant Human PGLS protein (His tag N-Terminus)

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Recombinant Human PGLS protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 258 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA.

別名を表示する

6-phosphogluconolactonase, 6PGL, PGLS

1 Images
SDS-PAGE - Recombinant Human PGLS protein (His tag N-Terminus) (AB101059)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PGLS protein (His tag N-Terminus) (AB101059)

15% SDS-PAGE showing ab10105 (3 μg)

Key facts

精製度

>95% SDS-PAGE

ab101059 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, ELISA

applications

生物活性

No

アクセッション番号

O95336

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

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Proteins & Peptides

AB126671

Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus)

SDS-PAGE - Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus) (AB126671)

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Primary Antibodies

AB180610

Anti-TLR8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB180610)

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Primary Antibodies

AB229980

Anti-PGLS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (AB229980)

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Proteins & Peptides

AB161165

Recombinant Human WASF3 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human WASF3 protein (GST tag N-Terminus) (AB161165)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL","proteinLength":"Full Length","predictedMolecularWeight":"29.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95336","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PGLS also known as 6-phosphogluconolactonase operates in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone into 6-phosphogluconate. This enzyme has an approximate mass of 27 kDa. PGLS expresses widely in various tissues but it is especially abundant in liver and adipose tissues where the pentose phosphate pathway activity is high.
Biological function summary

6-phosphogluconolactonase plays a role in cellular metabolism by ensuring the smooth progression of the pentose phosphate pathway. This pathway is important for the production of NADPH which acts as a reducing agent in several biosynthetic reactions. PGLS does not form part of a multiprotein complex but serves a singular enzymatic role essential for maintaining cellular redox state.

Pathways

The pentose phosphate pathway lies at the center of PGLS's function in its role of maintaining NADPH levels for reductive biosynthesis and protection against oxidative stress. In this pathway PGLS closely associates with glucose-6-phosphate dehydrogenase (G6PD) which provides the substrate for PGLS. Both play a sequential role in the oxidative branch of the pentose phosphate pathway influencing cellular processes such as fatty acid synthesis and ribose-5-phosphate production.

PGLS connects to the metabolic and oxidative balance within cells indirectly affecting conditions like cancer and metabolic disorders. Alterations in its activity or expression could lead to dysregulation of NADPH levels linking it with oxidative stress-related pathologies. PGLS's functional relation to G6PD could influence its involvement in diseases where altered glucose metabolism and the oxidative state are disturbed.
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一般的な情報

機能

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.

配列の類似性

Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.

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製品プロトコール

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ターゲットの情報

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
See full target information PGLS
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