Recombinant Human PFTK1 protein (ab167928)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human PFTK1 protein -
精製度
> 95 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MHGYFGCNAA AEPGYSAFVG TPQICVTKMS TRNCQGMDSV IKPLDTIPED KKVRVQRTQS TFDPFEKPAN QVKRVHSENN ACINFKTSST GKESPKVRRH SSPSSPTSPK FGKADSYEKL EKLGEGSYAT VYKGKSKVNG KLVALKVIRL QEEEGTPFTA IREASLLKGL KHANIVLLHD IIHTKETLTL VFEYVHTDLC QYMDKHPGGL HPDNVKLFLF QLLRGLSYIH QRYILHRDLK PQNLLISDTG ELKLADFGLA RAKSVPSHTY SNEVVTLWYR PPDVLLGSTE YSTCLDMWGV GCIFVEMIQG VAAFPGMKDI QDQLERIFLV LGTPNEDTWP GVHSLPHFKP ERFTLYSSKN LRQAWNKLSY VNHAEDLASK LLQCSPKNRL SAQAALSHEY FSDLPPRLWE LTDMSSIFTV PNVRLQPEAG ESMRAFGKNN SYGKSLSNSK H -
予測される分子量
72 kDa including tags -
領域
1 to 451 -
タグ
proprietary tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab167928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
関連情報
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別名
- cdk14
- CDK14_HUMAN
- Cell division protein kinase 14
see all -
機能
Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. -
組織特異性
Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cell membrane. Cytoplasm. Nucleus. Recruited to the cell membrane by CCNY. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human PFTK1 protein (ab167928)SDS-PAGE analysis of ab167928.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab167928 は 1 報の論文で使用されています。
- Lu F et al. Upregulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/ß-catenin signaling pathway. World J Surg Oncol 18:147 (2020). PubMed: 32605563