Recombinant Human PEDF protein (ab287924)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Suitable for: SDS-PAGE, MS, HPLC
製品の詳細
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製品名
Recombinant Human PEDF protein
PEDF タンパク質・ペプチド 製品一覧 -
精製度
>= 95 % SDS-PAGE.
>= 95 % HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVR SSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIH GTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVL TGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVT KFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLT GSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKL SYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGA GTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP -
予測される分子量
44 kDa -
実際の分子量
44 kDa -
分子量情報
Mass determination by ESI TOF: Predicted MW is 44444.85 +/- 10 Da. Observed MW is 44446.67 -
領域
20 to 418 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
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特性
Our Abpromise guarantee covers the use of ab287924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose -
再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
関連情報
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別名
- Cell proliferation-inducing gene 35 protein
- EPC 1
- EPC-1
see all -
機能
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
組織特異性
Retinal pigment epithelial cells and blood plasma. -
配列類似性
Belongs to the serpin family. -
発生段階
Expressed in quiescent cells. -
ドメイン
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
翻訳後修飾
The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human PEDF protein (ab287924)
SDS-PAGE analysis of ab287924
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Mass Spectrometry - Recombinant Human PEDF protein (ab287924)Mass determination by ESI TOF: 44444.85 +/- 10Da
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HPLC - Recombinant Human PEDF protein (ab287924)HPLC analysis of ab287924
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab287924 は論文での使用が確認できていません。
