AB123157

Recombinant Human PCYT2 protein (His tag N-Terminus)

Name: Recombinant Human PCYT2 protein (ab123157) | Abcam
Brand: Abcam Limited
SKU: AB123157
Price: 88000 JPY
Availability: InStock

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Recombinant Human PCYT2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 389 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

Ethanolamine-phosphate cytidylyltransferase, CTP:phosphoethanolamine cytidylyltransferase, Phosphorylethanolamine transferase, PCYT2

1 Images

SDS-PAGE - Recombinant Human PCYT2 protein (His tag N-Terminus) (AB123157)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PCYT2 protein (His tag N-Terminus) (AB123157)

15% SDS-PAGE analysis of 3 µg of Human PCYT2 protein (ab123157).

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

アクセッション番号

Q99447

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDEEIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQPGETVIYVAGAFDLFHIGHVDFLEKVHRLAERPYIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRYVSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRIITNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF","proteinLength":"Full Length","predictedMolecularWeight":"45.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":389,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99447","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PCYT2 also known as ethanolamine-phosphate cytidylyltransferase is an enzyme that plays a critical role in phospholipid metabolism. It has a molecular mass of approximately 41 kDa. PCYT2 is expressed in various tissues with high levels found in the liver and brain. The enzyme catalyzes the conversion of phosphoethanolamine and CTP to CDP-ethanolamine an important step in the CDP-ethanolamine pathway which synthesizes phosphatidylethanolamine.

Biological function summary

The function of PCYT2 centers around lipid metabolism and membrane biosynthesis. PCYT2 acts as an integral component of the CDP-ethanolamine pathway which is essential for the production of phosphatidylethanolamine a principal phospholipid in cell membranes. It works in conjunction with other enzymes to ensure the proper biosynthesis of phospholipids. Although its precise participation as part of a larger protein complex remains uncertain its enzymatic activity is significant for lipid homeostasis.

Pathways

The activity of PCYT2 mainly fits into the phosphatidylserine decarboxylation and CDP-ethanolamine pathways. The enzyme interacts with CDP-diacylglycerol and phosphatidylcholine playing a significant role in lipid assembly and remodeling of cellular membranes. It shares functional interactions within these pathways with other enzymes like CHPT1 which further converges toward the regulation of phosphatidylcholine and phosphatidylethanolamine synthesis.

Mutations in PCYT2 have been linked to neurodegenerative diseases and certain metabolic disorders. Its dysfunction can contribute to neurological symptoms due to its fundamental role in maintaining normal brain phospholipid levels. The protein's connections to disorders particularly concerning phospholipid imbalance include interactions with related enzymes like EPT1. Such connections underline how PCYT2 disruptions can impact phospholipid biosynthesis and potential disease outcomes.

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製品の性状

製品の状態

Liquid

補足情報

ab123157 was purified using conventional chromatography.

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一般的な情報

機能

Ethanolamine-phosphate cytidylyltransferase that catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PubMed : 31637422, PubMed : 9083101). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PubMed : 9083101).

配列の類似性

Belongs to the cytidylyltransferase family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information PCYT2

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Abcam product promise

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Recombinant Human PCYT2 protein (His tag N-Terminus)