AB51234

Recombinant Human Parathyroid Hormone protein (Tag Free)

Name: Recombinant Human Parathyroid Hormone protein (ab51234) | Abcam
Brand: Abcam Limited
SKU: AB51234
Price: 59000 JPY
Availability: InStock

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(1 Publication)

Recombinant Human Parathyroid Hormone protein (Tag Free) is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, ELISA.

別名を表示する

Parathyroid hormone, PTH, Parathormone, Parathyrin

3 Images

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

sELISA

Unknown

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

Standard curve for Parathyroid Hormone (Analyte : ab51234); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml.

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Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

All lanes:

Western blot - Anti-Parathyroid Hormone antibody (<a href='/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (ab51234) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

true

Exposure time: 4min

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

ab51234 on 15%SDS-PAGE

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE, WB, ELISA

applications

生物活性

アクセッション番号

P01270

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 0.0584% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab51234 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (<a href='/products/primary-antibodies/parathyroid-hormone-antibody-a1-70-ab14493'>ab14493</a>) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml." } } }

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配列情報

[{"sequence":"MSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ","proteinLength":"Full Length","predictedMolecularWeight":"9.55 kDa","actualMolecularWeight":null,"aminoAcidEnd":115,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01270","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

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製品の性状

製品の状態

Liquid

補足情報

Human Parathyroid Hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

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一般的な情報

機能

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).

配列の類似性

Belongs to the parathyroid hormone family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Parathyroid hormone

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Microbial cell factories 23:273 PubMed39390566

2024

Secretion of the human parathyroid hormone through a microcin type I secretion system in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Flórez,Juan Marizcurrena,Magela Laviña,María F Azpiroz

View all publications

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Recombinant Human Parathyroid Hormone protein (Tag Free)