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AB152231

Recombinant Human Osteocalcin protein (GST tag N-Terminus)

Recombinant Human Osteocalcin protein (GST tag N-Terminus)

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(1 Review)

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(1 Publication)

Recombinant Human Osteocalcin protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 100 aa range, expressed in Wheat germ, with >80%, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP, BGLAP

1 Images
SDS-PAGE - Recombinant Human Osteocalcin protein (GST tag N-Terminus) (AB152231)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Osteocalcin protein (GST tag N-Terminus) (AB152231)

12.5% SDS-PAGE analysis of ab152231 stained with Coomassie Blue.

Key facts

精製度

>80%

Glutathione Sepharose

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

ELISA, SDS-PAGE, WB

applications

生物活性

No

アクセッション番号

P02818

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB309521

Anti-Osteocalcin antibody [EPR24665-63]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Osteocalcin antibody [EPR24665-63] (AB309521)

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Primary Antibodies

AB209484

Anti-Sp7 / Osterix antibody [EPR21034]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (AB209484)

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Primary Antibodies

AB192256

Anti-RUNX2 antibody [EPR14334]

Recombinant|Advanced Validation|RabMAb|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR14334] (AB192256)

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Proteins & Peptides

AB259381

Recombinant human IL-6 protein (Active)

Premium Bioactive

Functional Studies (Neut/Block) - Recombinant human IL-6 protein (Active) (AB259381)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MRALTLLALLALAALCIAGQAGAKPSGAESSKGAAFVSKQEGSEVVKRPRRYLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":100,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P02818","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification GST Tag
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.
Biological function summary

Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.

Pathways

Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).

Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.
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一般的な情報

機能

The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein (PubMed : 3019668, PubMed : 6967872). Acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization (By similarity). Binds strongly to apatite and calcium upon gamma-carboxylation; this modification is essential for bone metabolism (PubMed : 6967872, PubMed : 39880952).. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes : acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development : osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.

配列の類似性

Belongs to the osteocalcin/matrix Gla protein family.

翻訳後修飾

Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium (PubMed:6967872, PubMed:39880952). Decarboxylation promotes the hormone activity (By similarity).

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製品プロトコール

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ターゲットの情報

The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein (PubMed : 3019668, PubMed : 6967872). Acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization (By similarity). Binds strongly to apatite and calcium upon gamma-carboxylation; this modification is essential for bone metabolism (PubMed : 6967872, PubMed : 39880952).. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes : acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development : osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.
See full target information BGLAP
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:16070 PubMed29167431

2017

Fabrication and Verification of Conjugated AuNP-Antibody Nanoprobe for Sensitivity Improvement in Electrochemical Biosensors.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Khashayar,Ghassem Amoabediny,Bagher Larijani,Morteza Hosseini,Jan Vanfleteren
View all publications
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