AB270077

Recombinant human Oncostatin M/OSM protein (Active)

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Recombinant human Oncostatin M/OSM protein (Active) is a Human Full Length protein, in the 26 to 221 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

別名を表示する

Oncostatin-M, OSM

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FuncS

Supplier Data

Functional Studies - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.

ED₅₀ is ≤ 0.2ng/mL, corresponding to a specific activity of 5.00 x 10⁶ units/mg.

Mass Spectrometry - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

(M - 2Arg) + 0.8 Da (expected mass after Arg loss due to transpeptidation 21896 Da; +16 Da : Hydroxylation/Oxidation, +32 Da : dioxidation; +203 Da HexNAc, + 365 Da Hex(1)HexNAc(1).

Calc MW is 22208.41 Da.

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

(M - 2Arg) + 0.8 Da (expected mass after Arg loss due to transpeptidation 21896 Da; +16 Da : Hydroxylation/Oxidation, +32 Da : dioxidation; +203 Da HexNAc, + 365 Da Hex(1)HexNAc(1)

Calc MW is 22208.41 Da.

SDS-PAGE - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

SDS-PAGE - Recombinant human Oncostatin M/OSM protein (Active) (ab270077).

Purity ≥95%.

HPLC - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

HPLC

Supplier Data

HPLC - Recombinant human Oncostatin M/OSM protein (Active) (AB270077)

Purity by HPLC ≥95%.

Key facts

精製度

>95% SDS-PAGE

Purity =95% by HPLC.

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

アプリケーション

Mass Spec, FuncS, Cell Culture, HPLC, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.

ED₅₀ is ≤ 0.2ng/mL, corresponding to a specific activity of 5.00 x 10⁶ units/mg.

アクセッション番号

P13725

アニマルフリー

Yes

キャリアフリー

種

Human

再構成

Reconstitute with Phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

バッファー組成

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.

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配列情報

[{"linker":null,"sequence":"AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRR","proteinLength":"Full Length","predictedMolecularWeight":"22.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":221,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13725","tags":[]}]

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出荷温度及び保存条件

製品の状態

Lyophilized

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

-20°C|Ambient

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Oncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.

Biological function summary

Oncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.

Pathways

Oncostatin M involves itself in significant pathways like the JAK/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.

Oncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.

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一般的な情報

機能

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).

配列の類似性

Belongs to the LIF/OSM family.

翻訳後修飾

Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information OSM

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Recombinant human Oncostatin M/OSM protein (Active)