AB140060

Recombinant Human Oct4 protein (denatured) (His tag N-Terminus)

Name: Recombinant Human Oct4 protein (denatured) (ab140060) | Abcam
Brand: Abcam Limited
SKU: AB140060
Price: 100000 JPY
Availability: InStock

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Recombinant Human Oct4 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 265 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

別名を表示する

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

1 Images

SDS-PAGE - Recombinant Human Oct4 protein (denatured) (His tag N-Terminus) (AB140060)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Oct4 protein (denatured) (His tag N-Terminus) (AB140060)

15% SDS-PAGE analysis of ab140060 (3μg)

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

Q01860

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMHFYRLFLGATRRFLNPEWKGEIDNWCVYVLTSLLPFKIQSQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN","proteinLength":"Full Length","predictedMolecularWeight":"32.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":265,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q01860","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.

Biological function summary

Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.

Pathways

Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.

Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.

配列の類似性

Belongs to the POU transcription factor family. Class-5 subfamily.

翻訳後修飾

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information POU5F1

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Abcam product promise

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Recombinant Human Oct4 protein (denatured) (His tag N-Terminus)