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Recombinant Human NUDT21/CFIM25 protein (ab104669)

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SDS-PAGE - Recombinant Human NUDT21/CFIM25 protein (ab104669)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    製品の詳細

    • 製品名

      Recombinant Human NUDT21/CFIM25 protein

    • 精製度

      > 90 % SDS-PAGE.
      ab104669 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

    • 発現系

      Escherichia coli

    • アクセッション番号

      O43809

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGSSHHHHHHSSGLVPRGSHMSVVPPNRSQTGWPRGVTQFGNKYIQQTKP LTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTV EGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILG RQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQ EKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN

      • 予測される分子量

        28 kDa including tags

      • 領域

        1 to 227

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab104669 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Mass Spectrometry

    • 質量分析

      MALDI-TOF

    • 製品の状態

      Liquid

    • 備考

       This product was previously labelled as NUDT21

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0308% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride

    関連情報

    • 別名

      • CFIM 25
      • CFIM25
      • Cleavage and polyadenylation specific factor 5
      • Cleavage and polyadenylation specific factor 5 25 kD subunit
      • Cleavage and polyadenylation specific factor 5 25 kDa
      • Cleavage and polyadenylation specificity factor 25 kDa subunit
      • Cleavage and polyadenylation specificity factor subunit 5
      • Cleavage factor Im complex 25 kDa subunit
      • CPSF 25
      • CPSF 25 kDa subunit
      • CPSF 5
      • CPSF25
      • CPSF5
      • CPSF5_HUMAN
      • DKFZp686H1588
      • Nucleoside diphosphate linked moiety X motif 21
      • Nucleoside diphosphate-linked moiety X motif 21
      • Nudix (nucleoside diphosphate linked moiety X) type motif 21
      • Nudix hydrolase 21
      • Nudix motif 21
      • NUDT 21
      • Nudt21
      • Pre mRNA cleavage factor Im (25kD)
      • Pre mRNA cleavage factor Im 25 kDa subunit
      • Pre mRNA cleavage factor Im 25kD subunit
      • Pre-mRNA cleavage factor Im 25 kDa subunit
      see all

    • 機能

      Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.

    • 配列類似性

      Belongs to the Nudix hydrolase family. CPSF5 subfamily.
      Contains 1 nudix hydrolase domain.

    • 翻訳後修飾

      Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.

    • 細胞内局在

      Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human NUDT21/CFIM25 protein (ab104669)
      SDS-PAGE - Recombinant Human NUDT21/CFIM25 protein (ab104669)
      15% SDS-PAGE showing ab104669 at approximately 28.3kDa (3µg).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab104669 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab104669 は 1 報の論文で使用されています。

    • Chatrikhi R  et al. RNA Binding Protein CELF2 Regulates Signal-Induced Alternative Polyadenylation by Competing with Enhancers of the Polyadenylation Machinery. Cell Rep 28:2795-2806.e3 (2019). PubMed: 31509743

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