AB101042

Recombinant Human NUCB2 protein

Name: Recombinant Human NUCB2 protein (ab101042) | Abcam
Brand: Abcam Limited
SKU: AB101042
Price: 118000 JPY
Availability: InStock

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Recombinant Human NUCB2 protein is a Human Fragment protein, in the 25 to 106 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

別名を表示する

NEFA, NUCB2, Nucleobindin-2, DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Prepronesfatin

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SDS-PAGE - Recombinant Human NUCB2 protein (AB101042)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NUCB2 protein (AB101042)

14% SDS-PAGE analysis of ab101042
Lane 1 : Molecular weight markers; 14, 21, 31, 45, 66, 97 kDa
Lane 2 : ab101042 (reduced and heated, 5μg/lane)
Lane 3 : ab101042 (non-reduced and non-heated, 5μg/lane)

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, WB

applications

生物活性

アクセッション番号

P80303

アニマルフリー

キャリアフリー

種

Human

再構成

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

バッファー組成

Constituents: 0.29% Sodium chloride, 0.242% Tris

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C.

Previously labelled as Nucleobindin 2.

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配列情報

[{"sequence":"MKHHHHHHASVPIDIDKTKVQNIHPVESAKIEPPDTGLYYDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTKLDEL","proteinLength":"Fragment","predictedMolecularWeight":"10.79 kDa","actualMolecularWeight":null,"aminoAcidEnd":106,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P80303","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NUCB2 also known as nesfatin-1 precursor is a calcium-binding protein involved in various physiological processes. This 50-kDa protein integrates signals related to energy balance and is important for appetite regulation. NUCB2 is expressed in several tissues such as the hypothalamus pancreas and adipose tissue which suggests its involvement in widespread bodily functions. The protein is produced as an 82-kDa precursor protein cleaved to release active fragments like nesfatin-1 which possesses biological activity.

Biological function summary

This protein influences food intake and energy homeostasis. NUCB2 is part of a signaling complex that modulates cellular responses to metabolic needs. It functions as a satiety molecule signaling when the body does not require additional food intake. The protein's regulatory role in appetite makes it important for maintaining body weight and energy utilization. NUCB2's expression in specific brain areas particularly indicates its role in central nervous system pathways that control energy use.

Pathways

NUCB2 engages in vital neural circuits governing appetite and energy expenditure. It connects with the melanocortin and neuropeptide Y pathways which are central to maintaining energy balance and body weight. The interaction of NUCB2 with neuropeptide Y an essential regulator of hunger exemplifies how it modulates these pathways despite its main role not being in leptin signaling.

Disturbances in NUCB2 are linked to obesity and stress-related conditions. Obesity involves disrupted signaling where NUCB2's role as a satiety signal becomes critical. The protein also plays a part in stress-related pathways influencing stress-induced appetite changes which can relate to conditions like depression. Alterations in NUCB2 expression or function can lead to abnormal body weight and stress responses making it a potential target for therapeutic interventions in these conditions.

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製品の性状

製品の状態

Lyophilized

補足情報

Purity as determined by densitometric image analysis: > 95%

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一般的な情報

機能

Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).. Nesfatin-1. Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

配列の類似性

Belongs to the nucleobindin family.

細胞内局在性

Nucleus envelope

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information NUCB2

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Abcam product promise

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Recombinant Human NUCB2 protein