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Recombinant Human Nodal protein (ab114307)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human Nodal protein (ab114307)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human Nodal protein

    • 発現系

      Wheat germ

    • アクセッション番号

      Q96S42

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSML YVDNGRVLLDHHKDMIVEECGC

      • 予測される分子量

        34 kDa including tags

      • 領域

        275 to 346

      • タグ

        GST tag N-Terminus

    関連製品

    特性

    Our Abpromise guarantee covers the use of ab114307 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • HTX5
      • MGC138230
      • Nodal
      • Nodal Growth Differentiation Factor
      • Nodal homolog
      • nodal homolog (mouse)
      • Nodal, Mouse, Homolog
      • NODAL_HUMAN
      see all

    • 機能

      Essential for mesoderm formation and axial patterning during embryonic development.

    • 関連疾患

      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.

    • 配列類似性

      Belongs to the TGF-beta family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Nodal protein (ab114307)
      SDS-PAGE - Recombinant Human Nodal protein (ab114307)
      12.5% SDS-PAGE image showing ab114307 Stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab114307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab114307 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Hello tehnical support,

    our customer is interested in Nodal protein that could be used for in vivo application in mice.

    They asked if the following AbCam products could be use for this kind of experiments?

    Nodal protein (ab114307)


    Nodal peptide (ab39951)


    Nodal peptide (ab39952)

    Thank You in advance.

    Best regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    With regards to our recombinant protein (ab114307), could you please confirm the application(s) you wish to use the product in? I can confirm that this particular recombinant protein can be used in antibody production. However, we have not tested the recombinant proteins for any activity assay yet so we do not know if the proteins are active or functional. We don’t produce the protein with endotoxin free system; therefore it is not suitable for using in cell culture system.

    We will contact you again regarding the other two products (ab39951 and ab39952) very shortly.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

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