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AB107700

Recombinant human NLK protein

Recombinant human NLK protein

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Recombinant human NLK protein is a Human Full Length protein, in the 1 to 527 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

LAK1, NLK, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1

2 Images
Functional Studies - Recombinant human NLK protein (AB107700)
  • FuncS

Unknown

Functional Studies - Recombinant human NLK protein (AB107700)

Specific activity of ab107700 was determined to be 2.4 nmol/min/mg.

SDS-PAGE - Recombinant human NLK protein (AB107700)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NLK protein (AB107700)

SDS-PAGE analysis of ab107700

Key facts

精製度

>85% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

FuncS, SDS-PAGE, WB

applications

生物活性

Yes

生物学的活性

Specific activity: 2.4 nmol/min/mg

アクセッション番号

Q9UBE8

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":527,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UBE8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NLK (Nemo-Like Kinase) functions as a serine/threonine protein kinase involved in various signaling pathways. It alternatively is known as nemo-like protein or nlk. The molecular mass of NLK is approximately 54 kDa. Expression of NLK occurs in various tissues but it is highly observed in the brain and testis. This wide expression suggests that NLK may have diverse functional roles.
Biological function summary

NLK participates in regulating cellular processes such as cell cycle apoptosis and transcription. It is not part of a larger complex but works within signaling pathways to influence these processes. NLK modulates the activity of several transcription factors particularly in the Wnt/beta-catenin signaling pathway where it negatively regulates the signaling to control gene expression relevant to cell proliferation and differentiation.

Pathways

NLK is an important player in the Wnt/beta-catenin and MAPK pathways. In the Wnt/beta-catenin pathway it associates with proteins such as TCF/LEF and beta-catenin acting to modulate transcription by phosphorylating these molecules. The MAPK pathway involves NLK in response to stress signals coordinating with proteins like TAK1 to mediate cellular responses. These pathways illustrate NLK's role in transmitting signals that govern cell fate and stress responses.

NLK has links to cancer and neurodegenerative diseases. Alterations in NLK expression or function can contribute to the development and progression of cancers by affecting the regulation of cell proliferation and apoptosis. Specifically dysregulation in the Wnt/beta-catenin pathway in which NLK acts relates to colorectal cancers. In the context of neurodegenerative diseases NLK has connections with the protein TDP-43 which is implicated in diseases such as amyotrophic lateral sclerosis highlighting its role in neuronal health and potential links to neuronal degradation.
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製品の性状

製品の状態

Liquid

補足情報

The purity was determined to be >85% by densitometry.GST Affinity Column purified.

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一般的な情報

機能

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).

配列の類似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

翻訳後修飾

Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).
See full target information NLK
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