Recombinant human NKp30 protein (Fc Chimera Active) (ab184896)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant human NKp30 protein (Fc Chimera Active) -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized Human B7-H6, His Tag at 10 μg/mL (100 μL/well) can bind ab184896 with a linear range of 2-78 ng/mL.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
LWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVVPGKEVRN GTPEFRGRLA PLASSRFLHDHQAELHIRDVRGHDASIYVCRVEVLGLG VGTGNGTRLVVEKEHPQLG -
予測される分子量
40 kDa including tags -
領域
19 to 135 -
タグ
Fc tag C-Terminus -
配列の追加情報
Extracellular domain of NCR3 fused with Fc fragment of Human IgG1 at the C-terminus.
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特性
Our Abpromise guarantee covers the use of ab184896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
ELISA
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製品の状態
Lyophilized -
備考
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.4
Constituents: 0.75% Glycine, 0.605% Tris, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- 1C7
- Activating natural killer receptor p30
- Activating NK A1 receptor
see all -
機能
Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. -
組織特異性
Selectively expressed by all resting and activated NK cells and weakly expressed in spleen. -
配列類似性
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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ELISA - Recombinant human NKp30 protein (Fc Chimera) (ab184896)
Immobilized Human B7-H6, His Tag at 10 μg/mL (100 μL/well) can bind ab184896 with a linear range of 2-78 ng/mL.
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SDS-PAGE - Recombinant human NKp30 protein (Fc Chimera) (ab184896)SDS-PAGE of reduced ab184896 stained overnight with Coomassie Blue. The protein migrates as 50-58 kDa due to glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184896 は論文での使用が確認できていません。