AB9796

Recombinant human NGF protein (Active)

Bioactive
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Recombinant human NGF protein (Active) is a Human Full Length protein, in the 122 to 241 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

別名を表示する

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

Key facts

精製度

>98% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE, FuncS, HPLC

applications

生物活性

Yes

生物学的活性

Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.

The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

アクセッション番号

P01138

アニマルフリー

キャリアフリー

種

Human

再構成

Reconstitute in water

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

style

left-column

style

left-column

配列情報

[{"sequence":"SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA","proteinLength":"Full Length","predictedMolecularWeight":"14 kDa","actualMolecularWeight":null,"aminoAcidEnd":241,"aminoAcidStart":122,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01138","tags":[]}]

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

style

left-column

style

left-column

製品の性状

製品の状態

Lyophilized

補足情報

>=98% HPLC analyses.Sterile filtered.

style

left-column

一般的な情報

機能

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

配列の類似性

Belongs to the NGF-beta family.

細胞内局在性

Endosome lumen

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information NGF

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant human NGF protein (Active)