AB112396

Recombinant Human NFAT5 protein (GST tag N-Terminus)

Name: Recombinant Human NFAT5 protein (ab112396) | Abcam
Brand: Abcam Limited
SKU: AB112396
Price: 118000 JPY
Availability: InStock

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Recombinant Human NFAT5 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1422 to 1531 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

KIAA0827, TONEBP, NFAT5, Nuclear factor of activated T-cells 5, NF-AT5, T-cell transcription factor NFAT5, Tonicity-responsive enhancer-binding protein, TonE-binding protein, TonEBP

1 Images

SDS-PAGE - Recombinant Human NFAT5 protein (GST tag N-Terminus) (AB112396)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NFAT5 protein (GST tag N-Terminus) (AB112396)

12.5% SDS-PAGE analysis of NFAT5 protein (ab112396), stained with Coomassie Blue.

Key facts

発現系

Wheat germ

アプリケーション

SDS-PAGE, ELISA, WB

applications

生物活性

アクセッション番号

O94916

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"(recombinant protein)." } } }

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配列情報

[{"sequence":"FHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF","proteinLength":"Fragment","predictedMolecularWeight":"37.73 kDa","actualMolecularWeight":null,"aminoAcidEnd":1531,"aminoAcidStart":1422,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O94916","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NFAT5 also known as TonEBP (tonicity-responsive enhancer binding protein) is a transcription factor belonging to the nuclear factor of activated T-cells (NFAT) family. It is involved in mechanistic functions through its role in the osmoregulatory mechanism aiding cells to adapt to changes in osmotic stress by regulating gene expression related to maintaining ionic and osmotic balance. NFAT5 has a molecular mass of approximately 170 kDa and is primarily expressed in kidney medulla cells where osmotic stress is prevalent.

Biological function summary

NFAT5 helps regulate the cellular response to hypertonic stress in several tissues including the kidneys brain and immune cells. It acts as a transcriptional activator that induces the expression of osmoprotective genes such as the sodium/myo-inositol transporter and aldose reductase. NFAT5 functions not as part of a larger complex but as an independent transcription factor which directly binds to enhancers within target gene promotors playing a critical role in cellular adaptation to hyperosmotic conditions.

Pathways

NFAT5 plays a significant role in the hypertonicity-induced signaling pathway and the osmoprotective pathway. Through these it influences key cellular processes by modulating the transcription of genes necessary for adaptation to osmotic stress. It stands in relation to other NFAT family members like NFATc1 and NFATc2 but distinctively differs as it does not rely directly on calcium and calcineurin for its activation unlike its relatives involved in the calcineurin/NFAT pathway.

NFAT5 has implications in cardiovascular diseases and diabetes mellitus. It's involved in cellular responses to hypertonic stress in various disease states notably influencing the vascular smooth muscle cell function in hypertension. Additionally NFAT5's regulation of osmoprotective genes connects it to metabolic disorders like diabetes where osmotic stress responses are disturbed. Interactions between NFAT5 and other proteins like TFEB (Transcription Factor EB) occur during conditions related to metabolic disorders indicating its broad relevance in stress-related pathologies.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed : 10377394). Mediates the transcriptional response to hypertonicity (PubMed : 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed : 22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 34049076).

翻訳後修飾

Phosphorylated (PubMed:10377394). Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization (PubMed:21209322).. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
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ターゲットの情報

See full target information NFAT5

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Abcam product promise

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Recombinant Human NFAT5 protein (GST tag N-Terminus)