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AB323115

Recombinant Human NF2 / Merlin Protein Standard (His tag)

Recombinant Human NF2 / Merlin Protein Standard (His tag)

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Recombinant Human NF2 / Merlin Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.

別名を表示する

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

2 Images
Sandwich ELISA - Recombinant Human NF2 / Merlin Protein Standard (His tag) (AB323115)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human NF2 / Merlin Protein Standard (His tag) (AB323115)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human NF2 / Merlin Protein Standard (His tag) (AB323115)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NF2 / Merlin Protein Standard (His tag) (AB323115)

SDS-PAGE analysis of ab323115 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

Escherichia coli

タグ

His tag C-Terminus

アプリケーション

sELISA, SDS-PAGE

applications

生物活性

No

アクセッション番号

P35240

アニマルフリー

Yes

キャリアフリー

No

種

Human

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Assay Kits

AB317778

Human NF2/Merlin ELISA Kit

SimpleStep

Sandwich ELISA - Human NF2/Merlin ELISA Kit (AB317778)

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Primary Antibodies

AB109244

Anti-NF2 / Merlin antibody [EPR2573(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (AB109244)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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配列情報

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"39.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P35240","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification His Tag
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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一般的な情報

機能

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

翻訳後修飾

Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741). The dephosphorylation of Ser-518 favors the interaction with NOP53 (PubMed:21167305).. Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.

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製品プロトコール

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ターゲットの情報

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
See full target information NF2
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