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AB153787

Recombinant Human Nectin 3 protein

Recombinant Human Nectin 3 protein

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Recombinant Human Nectin 3 protein is a Human Fragment protein, in the 58 to 366 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

別名を表示する

CD113, PRR3, PVRL3, NECTIN3, Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3

1 Images
SDS-PAGE - Recombinant Human Nectin 3 protein (AB153787)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Nectin 3 protein (AB153787)

SDS-PAGE analysis showing staining of ab153787.
Predicted MW 35 kDa.

Key facts

精製度

>95% SDS-PAGE

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Lyophilized from a 0.2 µM filtered solution.

エンドトキシンレベル

< 1 EU/µg

発現系

HEK 293 cells

タグ

His tag C-Terminus

アプリケーション

HPLC, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q9NQS3

アニマルフリー

No

キャリアフリー

No

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

storage-buffer

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組合せ製品

Proteins & Peptides

AB157982

Recombinant Human C1QA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human C1QA protein (GST tag N-Terminus) (AB157982)

  • 0
  • 0
Primary Antibodies

AB16913

Anti-Nectin 3 antibody [103-A1]

  • 4
  • 3
Proteins & Peptides

AB316640

Recombinant Human C4b Protein Standard (His tag)

Sandwich ELISA - Recombinant Human C4b Protein Standard (His tag) (AB316640)

  • 0
  • 0
Proteins & Peptides

AB199588

Recombinant Human C4 protein

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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配列情報

[{"linker":null,"sequence":"GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHQALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISAYNSVASLNCVDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"34.96 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":58,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9NQS3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Blue Ice
短期保存期間
A few weeks
短期保存温度
-20°C
長期保存温度
-20°C
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nectin-3 also known as CD113 or PVRL3 is an immunoglobulin-like cell adhesion molecule. It plays an important role in cell-cell adhesion by forming homo- and heterophilic trans-dimers with other nectins and nectin-like molecules. Nectin-3 has a molecular mass of approximately 83 kDa. Its expression is generally observed in neural tissues such as the brain as well as in testis and various epithelial tissues. This distribution allows it to contribute to diverse cellular interactions critical for tissue organization and development.
Biological function summary

Nectin-3 participates in the formation and maintenance of adherens junctions by interacting with cadherins and actin filaments. It is a member of the Nectin family which includes Nectin-1 Nectin-2 and Nectin-4 known for their roles in cell adhesion. The function of Nectin-3 is integral to the establishment of tight junctions contributing to the structural integrity and function of epithelial and endothelial cells. It often works as part of a complex with afadin which supports its connection to the actin cytoskeleton.

Pathways

Nectin-3 acts in the formation of synapses and is involved in signaling pathways related to cell adhesion such as the actin cytoskeleton regulation pathway. It plays a significant role in the activation of the Ras and Rac small GTPase signaling pathways facilitating changes in cytoskeletal dynamics during cell movement and morphogenesis. Nectin-3 closely associates with other proteins in the Nectin family such as Nectin-1 and Nectin-2 in these pathways enhancing intercellular communication.

Mutations or dysregulation of Nectin-3 have been linked to conditions like mental retardation and developmental abnormalities. It is particularly relevant in the context of intellectual disability where alterations in cell adhesion and signaling can impair proper neuronal connectivity and brain function. Furthermore Nectin-3 interacts with PVRL proteins highlighting its possible involvement in cancer progression and metastasis through altered cell adhesion properties.
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一般的な情報

機能

Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions (PubMed : 16216929). Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1 (PubMed : 16216929). Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation (PubMed : 16216929). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Also involved in the formation of cell-cell junctions, including adherens junctions and synapses (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1 : NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Plays a role in the morphology of the ciliary body (By similarity).

配列の類似性

Belongs to the nectin family.

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製品プロトコール

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ターゲットの情報

Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions (PubMed : 16216929). Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1 (PubMed : 16216929). Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation (PubMed : 16216929). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Also involved in the formation of cell-cell junctions, including adherens junctions and synapses (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1 : NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Plays a role in the morphology of the ciliary body (By similarity).
See full target information NECTIN3
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