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AB134886

Recombinant human Nanog protein

Recombinant human Nanog protein

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(2 Publications)

Recombinant human Nanog protein is a Human Full Length protein, in the 2 to 305 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

Tag free

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

DNA binding activity was demonstrated with ELISA using NANOG specific DNA binding oligo.

アクセッション番号

Q9H9S0

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: Glycerol (glycerin, glycerine), Potassium chloride, Sodium chloride, 3.4% DL-Arginine, 0.32% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2-8 µg/mL", "notes":"<p>For enhancing PiPS generation efficiency in combination with proteins Oct4, Sox2, Klf4 and cMyc.</p>" } } }
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配列情報

[{"sequence":"GSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDVESGGGGSPGRRRRRRRRRRR","proteinLength":"Full Length","predictedMolecularWeight":"36.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H9S0","tags":[]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nanog also known as Nanog homeobox is a transcription factor playing an important role in maintaining the pluripotency of embryonic stem cells. The molecular weight of Nanog is approximately 35 kDa. It is found mainly in the inner cell mass of the blastocyst but it also expresses in embryonic stem cells and some adult stem cell populations. Nanog acts by binding to DNA in a sequence-specific manner to regulate gene expression essential for stem cell self-renewal and pluripotency.
Biological function summary

Nanog plays a central role in stem cell biology operating as part of a complex regulatory network. Nanog interacts with other important transcription factors like Oct4 and Sox2 forming a core pluripotency network. This network suppresses the differentiation of stem cells by regulating the expression of genes involved in cell fate decisions. By modulating the expression of different pathways Nanog ensures the maintenance of an undifferentiated state in stem cells.

Pathways

Nanog is deeply embedded in the Wnt/β-Catenin and TGF-β signaling pathways important for stem cell maintenance and differentiation. In the Wnt/β-Catenin pathway Nanog works alongside proteins like β-catenin to drive the expression of genes that promote self-renewal. Meanwhile in the TGF-β pathway Nanog acts with proteins such as Smad2/3 to balance pluripotency and differentiation signals. These pathways critically support the complex network that sustains stem cell identity and function.

Nanog's expression relates closely to its role in various cancers such as glioblastoma and colorectal cancer. Abnormally high levels of Nanog contribute to the tumorigenicity of cancer cells by maintaining their self-renewal and undifferentiated state similar to its role in stem cells. Nanog interacts with proteins like p53 known for its tumor suppressor functions often leading to challenges in cancer treatment. Understanding Nanog's influence in these pathways provides valuable insights into therapeutic targets for combating cancer stem cell resilience.
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製品の性状

製品の状態

Liquid

補足情報

ab134886 was expressed in E. coli as inclusion bodies, refolded using “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.

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一般的な情報

機能

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

配列の類似性

Belongs to the Nanog homeobox family.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed : 25825768). Able to autorepress its expression in differentiating (ES) cells : binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
See full target information NANOG
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 39:110919 PubMed35675767

2022

Stub1 maintains proteostasis of master transcription factors in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Md Mahfuz Al Mamun,Muhammad Riaz Khan,Yifu Zhu,Yuwei Zhang,Shuai Zhou,Ran Xu,Ihtisham Bukhari,Rick F Thorne,Jinming Li,Xu Dong Zhang,Guangzhi Liu,Song Chen,Mian Wu,Xiaoyuan Song

Nature communications 12:3337 PubMed34099689

2021

High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ludovica Vanzan,Hadrien Soldati,Victor Ythier,Santosh Anand,Simon M G Braun,Nicole Francis,Rabih Murr
View all publications
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組合せ製品

Proteins & Peptides

AB316492

Recombinant Human/Mouse Oct4 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human/Mouse Oct4 Protein Standard (His tag) (AB316492)

  • 0
  • 0
Proteins & Peptides

AB169843

Recombinant human SOX2 protein

  • 0
  • 0
Primary Antibodies

AB214549

Anti-Nanog antibody [EPR20694] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR20694] - ChIP Grade (AB214549)

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

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