Recombinant Human NADPH oxidase 4 protein (ab289740)
Key features and details
- Expression system: Cell free
- Purity: >= 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human NADPH oxidase 4 protein
NADPH oxidase 4 タンパク質・ペプチド 製品一覧 -
精製度
>= 85 % SDS-PAGE. -
発現系
Cell free -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
MAVSWRSWLA NEGVKHLCLF IWLSMNVLLF WKTFLLYNQG PEYHYLHQML GLGLCLSRAS ASVLNLNCSL ILLPMCRTLL AYLRGSQKVP SRRTRRLLDK SRTFHITCGV TICIFSGVHV AAHLVNALNF SVNYSEDFVE LNAARYRDED PRKLLFTTVP GLTGVCMVVV LFLMITASTY AIRVSNYDIF WYTHNLFFVF YMLLTLHVSG GLLKYQTNLD THPPGCISLN RTSSQNISLP EYFSEHFHEP FPEGFSKPAE FTQHKFVKIC MEEPRFQANF PQTWLWISGP LCLYCAERLY RYIRSNKPVT IISVMSHPSD VMEIRMVKEN FKARPGQYIT LHCPSVSALE NHPFTLTMCP TETKATFGVH LKIVGDWTER FRDLLLPPSS QDSEILPFIQ SRNYPKLYID GPFGSPFEES LNYEVSLCVA GGIGVTPFAS ILNTLLDDWK PYKLRRLYFI WVCRDIQSFR WFADLLCMLH NKFWQENRPD YVNIQLYLSQ TDGIQKIIGE KYHALNSRLF IGRPRWKLLF DEIAKYNRGK TVGVFCCGPN SLSKTLHKLS NQNNSYGTRF EYNKESFS -
予測される分子量
70 kDa including tags -
領域
1 to 578 -
タグ
His tag N-Terminus -
配列の追加情報
10xHis
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特性
Our Abpromise guarantee covers the use of ab289740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate, 6% Trehalose
関連情報
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別名
- Kidney oxidase 1
- Kidney oxidase-1
- Kidney superoxide producing NADPH oxidase
see all -
機能
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. -
組織特異性
Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. -
配列類似性
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
発生段階
Expressed in fetal kidney and fetal liver. -
翻訳後修飾
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. -
細胞内局在
Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human NADPH oxidase 4 protein (ab289740)SDS-PAGE analysis of ab289740
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab289740 は論文での使用が確認できていません。
