JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB114325

Recombinant Human n-Myc/MYCN protein (GST tag N-Terminus)

Recombinant Human n-Myc/MYCN protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human n-Myc/MYCN protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 464 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

BHLHE37, NMYC, MYCN, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37

1 Images
SDS-PAGE - Recombinant Human n-Myc/MYCN protein (GST tag N-Terminus) (AB114325)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human n-Myc/MYCN protein (GST tag N-Terminus) (AB114325)

SDS-PAGE analysis of ab114325 on a 12.5% gel stained with Coomassie Blue.

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, ELISA, WB

applications

生物活性

No

アクセッション番号

P04198

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

組合せ製品

Proteins & Peptides

AB158935

Recombinant Human l-Myc protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human l-Myc protein (GST tag N-Terminus) (AB158935)

  • 0
  • 0
Proteins & Peptides

AB316529

Recombinant Human DLL3 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human DLL3 Protein Standard (His tag) (AB316529)

  • 0
  • 0
Proteins & Peptides

AB95932

Recombinant Human eIF2A protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human eIF2A protein (His tag N-Terminus) (AB95932)

  • 0
  • 0
Primary Antibodies

AB16898

Anti-n-Myc/MYCN antibody [NCM II 100]

Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (AB16898)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(for recombinant protein).</p>" } } }
style
left-column

製品の詳細

This product was previously labelled as n-Myc.
style
left-column

配列情報

[{"linker":null,"sequence":"MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC","proteinLength":"Full Length","predictedMolecularWeight":"77.11 kDa","actualMolecularWeight":null,"aminoAcidEnd":464,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P04198","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Proprietary technique
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

N-Myc also known as MYCN is a transcription factor encoded by the MYCN gene. It plays an important role in cell proliferation and differentiation. The n-Myc protein is part of the Myc family of transcription factors including c-Myc and c-Myb. n-Myc has a molecular weight of approximately 64 kDa. This protein expresses predominantly in developing tissues particularly in the brain but its expression is seen in other tissues during embryogenesis as well. This characteristic makes it important in early development and organ formation.
Biological function summary

N-Myc/MYCN functions in gene regulation by binding to E-box sequences in DNA facilitating transcription of genes essential for cellular growth and metabolism. n-Myc often forms a heterodimer with the MAX protein which enhances its DNA-binding capacity and transcriptional activity. This activity influences processes like apoptosis cell differentiation and cell cycle progression. The ncm mouse model a genetically engineered mouse with MYCN overexpression demonstrates the effects of deregulated n-Myc expression.

Pathways

MYCN significantly impacts the p53 signaling pathway and the Wnt signaling pathway. In the p53 signaling pathway n-Myc interacts with p53 and MDM2 regulating cell cycle checkpoints and apoptosis. In the Wnt pathway MYCN interplays with molecules like β-catenin to influence cell division and differentiation. These interactions highlight the role of n-Myc in maintaining cellular homeostasis and response to external signals that affect growth and division.

N-Myc is closely linked to neuroblastoma and medulloblastoma aggressive forms of pediatric cancers. MYCN amplification often indicates a poor prognosis in neuroblastoma playing a role in tumor progression and resistance to therapy. In medulloblastoma overexpression of n-Myc partners with proteins like Aurora A kinase to promote oncogenesis. Targeting these protein interactions offers potential therapeutic strategies for these MYCN-related cancers.
style
left-column

一般的な情報

機能

Positively regulates the transcription of MYCNOS in neuroblastoma cells.

翻訳後修飾

Phosphorylated by GSK3-beta which may promote its degradation (PubMed:24391509). Phosphorylated by AURKA (PubMed:27837025).

細胞内局在性

Nucleus

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Positively regulates the transcription of MYCNOS in neuroblastoma cells.
See full target information MYCN
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com