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AB60341

Recombinant ヒト MST4 protein (GST tag N-Terminus)

Recombinant human MST4 protein (GST tag N-Terminus)

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Recombinant human MST4 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

MASK, MST4, STK26, Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, Serine/threonine-protein kinase MASK, MST-4, STE20-like kinase MST4

4 Images
Functional Studies - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)
  • FuncS

Unknown

Functional Studies - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)
  • FuncS

Unknown

Functional Studies - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)

The specific activity of MST4 (ab60341) was determined to be 23 nmol/min/mg as per activity assay protocol

Western blot - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)
  • WB

Unknown

Western blot - Recombinant human MST4 protein (GST tag N-Terminus) (AB60341)

ab52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.

All lanes:

Western blot - Anti-MST4 antibody [EP1864Y] (<a href='/products/primary-antibodies/mst4-antibody-ep1864y-ab52491'>ab52491</a>) at 1/20000 dilution

All lanes:

Western blot - Recombinant human MST4 protein (GST tag N-Terminus) (ab60341) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 47 kDa

false

Exposure time: 20s

SDS-PAGE - Recombinant human MST4 protein (AB60341)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MST4 protein (AB60341)

SDS PAGE analysis of ab60341

Key facts

精製度

>90% SDS-PAGE

Purity: >90% as determined by densitometry. Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS, WB

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

Q9P289

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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組合せ製品

Primary Antibodies

AB52491

Anti-MST4 antibody [EP1864Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EP1864Y] (AB52491)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab60341 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/mst4-antibody-ep1864y-ab52491'>ab52491</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay: Use at an assay dependent dilution. Specific activity: 23 nmol/min/mg.</p>" } } }
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製品の詳細

ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9P289","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MST4 also known as mammalian sterile 20-like kinase 4 is a serine/threonine kinase with a molecular mass of approximately 53 kDa. It belongs to the germinal center kinase (GCK) family and is expressed in various tissues showing higher levels in the brain and testis. MST4 localizes to the Golgi apparatus where it plays a role in regulating cellular processes.
Biological function summary

MST4 contributes to cell growth apoptosis and stress response signaling. It does not operate alone; MST4 is a part of the STRIPAK complex. The protein involves itself in the regulation of the actin cytoskeleton thereby influencing cell motility and polarity. By modulating these processes MST4 can impact developmental and stress-induced cellular pathways.

Pathways

MST4 participates in the Hippo signaling pathway which regulates organ size by controlling cell proliferation and apoptosis. In this pathway MST4 interacts with other proteins such as MST1 and MST2 to execute its functions. MST4 also participates in the MAPK signaling pathway where it connects to various cellular stress responses contributing to the transcriptional activity of downstream effectors.

MST4 has implications in cancer and neurodegenerative diseases. Alterations in MST4 expression or activity have been associated with tumor progression in several cancers. Additionally it's linked to neurodegeneration through its interactions with proteins such as Tau where dysregulated pathways may lead to pathological conditions. These connections highlight the importance of MST4 in maintaining cellular homeostasis and the implications of its dysfunction in disease states.
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一般的な情報

機能

Serine/threonine-protein kinase that acts as a mediator of cell growth (PubMed : 11641781, PubMed : 17360971). Modulates apoptosis (PubMed : 11641781, PubMed : 17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed : 27807006). Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux (PubMed : 29232556). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).

配列の類似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase that acts as a mediator of cell growth (PubMed : 11641781, PubMed : 17360971). Modulates apoptosis (PubMed : 11641781, PubMed : 17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed : 27807006). Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux (PubMed : 29232556). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
See full target information STK26
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