AB323114

Recombinant human MMRN1/ECM Protein Standard (His tag)

Name: Recombinant human MMRN1/ECM Protein Standard (His tag) (ab323114) | Abcam
Brand: Abcam Limited
SKU: AB323114
Price: 29000 JPY
Availability: InStock

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Recombinant human MMRN1/ECM Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

別名を表示する

ECM, EMILIN4, GPIA*, MMRN, MMRN1, Multimerin-1, EMILIN-4, Elastin microfibril interface located protein 4, Endothelial cell multimerin, Elastin microfibril interfacer 4

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Sandwich ELISA - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

sELISA

Supplier Data

Sandwich ELISA - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MMRN1/ECM Protein Standard (His tag) (AB323114)

SDS-PAGE analysis of ab323114 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

HEK 293 cells

アプリケーション

SDS-PAGE, sELISA

applications

生物活性

アクセッション番号

Q13201

アニマルフリー

Yes

キャリアフリー

種

Human

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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配列情報

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q13201","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein.

翻訳後修飾

The N-terminus is blocked.. Extensively N-glycosylated.. O-fucosylated within the EMI domain (at Thr-216 and Thr-265) by POFUT3 and POFUT4 (PubMed:39775168). O-fucosylation at Thr-216 and Thr-1055 are required for facilitating protein folding and secretion (PubMed:38237698, PubMed:39775168).

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information MMRN1

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant human MMRN1/ECM Protein Standard (His tag)