Recombinant Human MMP9 protein (Proenzyme) (ab82955)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: ELISA, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human MMP9 protein (Proenzyme)
MMP9 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
20 to 707 -
タグ
His tag N-Terminus -
配列の追加情報
Corresponding to the pro form of the protein minus the signal peptide with a N-terminal 6X his tag.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab82955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.20
Constituents: PBS, 50% Glycerol
関連情報
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別名
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
機能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
組織特異性
Produced by normal alveolar macrophages and granulocytes. -
関連疾患
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
配列類似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
画像
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Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
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SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (ab82955)ab82955 on SDS-PAGE. -
Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab82955 は 2 報の論文で使用されています。
- Del Ben M et al. Overexpression of the Vitronectin V10 Subunit in Patients with Nonalcoholic Steatohepatitis: Implications for Noninvasive Diagnosis of NASH. Int J Mol Sci 19:N/A (2018). PubMed: 29463024
- Matou-Nasri S et al. Biological impact of advanced glycation endproducts on estrogen receptor-positive MCF-7 breast cancer cells. Biochim Biophys Acta Mol Basis Dis 1863:2808-2820 (2017). PubMed: 28712835