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Recombinant Human MKP-1 protein (ab114331)

Submit a review Q&A (1)References (1)
SDS-PAGE - Recombinant Human MKP-1 protein (ab114331)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, WB, ELISA

    製品の詳細

    • 製品名

      Recombinant Human MKP-1 protein

    • 発現系

      Wheat germ

    • アクセッション番号

      P28562

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MVMEVGTLDA GGLRALLGER AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM GLEHIVPNAE LRGRLLAGAY HAVVLLDERS AALDGAKRDG TLALAAGALC REARAAQVFF LKGGYEAFSA SCPELCSKQS TPMGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILPF LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKNA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHST NSALSYLQSP ITTSPSC

      • 予測される分子量

        66 kDa including tags

      • 領域

        1 to 367

    特性

    Our Abpromise guarantee covers the use of ab114331 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Western blot

      ELISA

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • CL 100
      • CL100
      • Dual Specificity Phosphatase 1
      • Dual specificity protein phosphatase 1
      • Dual specificity protein phosphatase hVH1
      • DUS1_HUMAN
      • DUSP 1
      • Dusp1
      • HVH1
      • MAP kinase phosphatase 1
      • Mitogen-activated protein kinase phosphatase 1
      • MKP-1
      • MKP1
      • Protein tyrosine phosphatase CL100
      • Protein-tyrosine phosphatase CL100
      • PTPN10
      • Serine/threonine specific protein phosphatase
      • VH1
      see all

    • 機能

      Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.

    • 組織特異性

      Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

    • 配列類似性

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 rhodanese domain.
      Contains 1 tyrosine-protein phosphatase domain.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human MKP-1 protein (ab114331)
      SDS-PAGE - Recombinant Human MKP-1 protein (ab114331)
      ab114331 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab114331 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab114331 は 1 報の論文で使用されています。

    • Hartleben G  et al. Combination therapies induce cancer cell death through the integrated stress response and disturbed pyrimidine metabolism. EMBO Mol Med 13:e12461 (2021). PubMed: 33665961

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Is this protein active?

    Read More
    Answer

    Thank you for contacting us. The activity for ab 113160 is as follows:

    Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

    The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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