Recombinant human MK2 protein (ab60307)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human MK2 protein
MK2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: 254 nmol/min/mg.
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精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
68 kDa -
領域
46 to 400 -
タグ
GST tag N-Terminus -
配列の追加情報
Recombinant fragment, corresponding to amino acids 46-end of Human MAPKAP Kinase 2.
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MAPKAP Kinase 2
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- MAP kinase activated protein Kinase 2
- MAP kinase-activated protein kinase 2
- MAPK activated protein kinase 2
see all -
機能
Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. -
組織特異性
Expressed in all tissues examined. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated and activated by MAP kinase. - Information by UniProt
画像
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Functional Studies - Recombinant human MK2 protein (ab60307)The specific activity of MK2 (ab60307) was determined to be 315 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human MK2 protein (ab60307)SDS PAGE analysis of ab60307 -
Functional Studies - Recombinant human MK2 protein (ab60307)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human MK2 protein (ab60307)ab60307 on SDS-PAGE.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab60307 は 1 報の論文で使用されています。
- Chen IT et al. Promyelocytic leukemia protein targets MK2 to promote cytotoxicity. EMBO Rep 22:e52254 (2021). PubMed: 34633746