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AB60307

Recombinant ヒト MK2 protein (GST tag N-Terminus)

Recombinant human MK2 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant human MK2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 46 to 400 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2, MAPKAPK2

4 Images
Functional Studies - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)
  • FuncS

Unknown

Functional Studies - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)
  • FuncS

Unknown

Functional Studies - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)

The specific activity of MK2 (ab60307) was determined to be 315 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)

ab60307 on SDS-PAGE.

SDS-PAGE - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MK2 protein (GST tag N-Terminus) (AB60307)

SDS PAGE analysis of ab60307

Key facts

精製度

>90% Densitometry

Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific activity: 254 nmol/min/mg.

アクセッション番号

P49137

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Proteins & Peptides

AB125663

Recombinant human p38 beta/MAPK11 protein (GST tag N-Terminus)

Functional Studies - Recombinant human p38 beta/MAPK11 protein (GST tag N-Terminus) (AB125663)

  • 0
  • 0
Proteins & Peptides

AB271732

Recombinant human RIP protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human RIP protein (Active) (GST tag N-Terminus) (AB271732)

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  • 0
Primary Antibodies

AB32567

Anti-MK2 antibody [E341]

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-MK2 antibody [E341] (AB32567)

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  • 1
Proteins & Peptides

AB104029

Recombinant human HDAC4 protein (GST tag N-Terminus)

Functional Studies - Recombinant human HDAC4 protein (AB104029)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay: Use at 1/4 dilution with 50 ng/ul BSA solution.</p>" } } }
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製品の詳細

ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity

This product was previously labelled as MAPKAP Kinase 2

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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"68 kDa","actualMolecularWeight":null,"aminoAcidEnd":400,"aminoAcidStart":46,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P49137","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The MK2 protein also known as mitogen-activated protein kinase-activated protein kinase 2 or MAPKAPK2 is an important enzyme involved in cellular signaling. MK2 has a protein mass of approximately 44 kDa. It is widely expressed in various tissues indicating its broad functional roles. MK2 interacts with MAP kinases facilitating the transmission of signals within cells during stress responses and inflammation.
Biological function summary

MK2 plays an important role in the cellular stress response by regulating cytokine production particularly in inflammation. It acts as part of a protein complex with p38 MAPK enhancing its kinase activity. This interaction allows MK2 to control the expression of inflammatory cytokines and other stress-responsive proteins. The complex also responds to cellular stress signals allowing it to modulate gene expression effectively.

Pathways

MK2 contributes to the p38 MAPK signaling pathway a major route for stress and inflammatory responses in cells. MK2 often interacts with related proteins like p38 MAPK which are directly upstream in the signaling cascade. Additionally MK2 is involved in the post-transcriptional regulation of mRNA stability and translation interfacing with the NF-kB signaling pathway highlighting its regulatory role in inflammation.

MK2's role in inflammation links it to numerous inflammatory diseases such as rheumatoid arthritis and Crohn's disease. MK2's interaction with other proteins like p38 MAPK and downstream effectors such as transcription factors emphasizes its therapeutic potential. Targeting MK2 and its interaction with these proteins may offer novel treatments for conditions characterized by dysregulated inflammatory responses.
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一般的な情報

機能

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed : 16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed : 26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization : following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

配列の類似性

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

翻訳後修飾

Sumoylation inhibits the protein kinase activity.. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.

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製品プロトコール

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ターゲットの情報

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed : 16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed : 26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization : following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
See full target information MAPKAPK2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 22:e52254 PubMed34633746

2021

Promyelocytic leukemia protein targets MK2 to promote cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

I-Ting Chen,Hsiao-Chi Chen,Yu-Hsun Lo,Peng-Yeh Lai,Fu-Yi Hsieh,Yung-Hsuan Wu,Hsiu-Ming Shih,Ming-Zong Lai
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