JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB307786

Recombinant Human MICA Protein (His-tag) (Active)

Recombinant Human MICA Protein (His-tag) (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human MICA Protein (His-tag) (Active) is a Human Fragment protein, in the 24 to 308 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec.

別名を表示する

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

4 Images
Biological Activity - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • Biological Activity

Lab

Biological Activity - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

Mass determination by ESI-TOF. Predicted MW is 34341.59 Da (+/-10 Da by ESI-TOF). Observed MW is 34348.97 Da.

SDS-PAGE - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

SDS-PAGE analysis of ab307786

HPLC - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)
  • HPLC

Supplier Data

HPLC - Recombinant Human MICA Protein (His-tag) (Active) (AB307786)

HPLC analysis of ab307786

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

His tag C-Terminus

アプリケーション

SDS-PAGE, Mass Spec, HPLC

applications

生物活性

Yes

生物学的活性

Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).

アクセッション番号

Q29983

アニマルフリー

Yes

キャリアフリー

No

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"sequence":"EPHSLRYNLTVLSWDGSVQSGFLTEVHLDGQPFLRCDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNLETKEWTMPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLKSGVVLRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQ","proteinLength":"Fragment","predictedMolecularWeight":"34.34 kDa","actualMolecularWeight":"34.35 kDa","aminoAcidEnd":308,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q29983","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.
Biological function summary

The MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.

Pathways

MICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.

MICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.
style
left-column

製品の性状

製品の状態

Lyophilized

補足情報

SDS-PAGE >= 95%

style
left-column

一般的な情報

機能

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

配列の類似性

Belongs to the MHC class I family. MIC subfamily.

翻訳後修飾

N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.. Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol.. N-glycosylation is necessary for cell surface expression.. (Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation.

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).
See full target information MICA
style
left-column
style
left-column
style
right-column

組合せ製品

Proteins & Peptides

AB307787

Recombinant Human MICB Protein (His-tag) (Active)

Premium Bioactive

Biological Activity - Recombinant Human MICB Protein (His-tag) (Active) (AB307787)

  • 0
  • 0
Primary Antibodies

AB224702

Anti-MICA + MICB antibody [EPR22031]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (AB224702)

  • 0
  • 0
Primary Antibodies

AB300485

Anti-MICB antibody [EPR26362-65]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-MICB antibody [EPR26362-65] (AB300485)

  • 0
  • 0
Assay Kits

AB59569

Human MICA ELISA Kit

ELISA - Human MICA ELISA Kit (AB59569)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com