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Recombinant Human MGMT protein (His tag) (ab185395)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

製品の詳細

  • 製品名

    Recombinant Human MGMT protein (His tag)
    MGMT タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % SDS-PAGE.
    Purity is determined by SEC-HPLC and reducing SDS-PAGE.

  • エンドトキシン・レベル

    < 1.000 Eu/µg

  • 発現系

    Escherichia coli

  • アクセッション番号

    P16455

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MGSSHHHHHHSSGLVPRGSHMDKDCEMKRTTLDSPLGKLELSGCEQGLHE IKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFP VPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAV GGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPG LGGSSGLAGAWLKGAGATSGSPPAGRN

    • 予測される分子量

      24 kDa including tags

    • 領域

      1 to 207

    • タグ

      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab185395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    HPLC

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris-HCl buffer, 0.03% EDTA

関連情報

  • 別名

    • 6 O methylguanine DNA methyltransferase
    • 6-O-methylguanine-DNA methyltransferase
    • Agat
    • AGT
    • AI267024
    • EC 2.1.1.63
    • Methylated DNA protein cysteine methyltransferase
    • Methylated-DNA--protein-cysteine methyltransferase
    • Methylguanine DNA methyltransferase
    • MGC107020
    • MGMT
    • MGMT_HUMAN
    • O 6 methylguanine DNA alkyltransferase
    • O 6 methylguanine DNA methyltransferase
    • O-6-methylguanine-DNA methyltransferase
    • O-6-methylguanine-DNA-alkyltransferase
    see all

  • 機能

    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

  • 配列類似性

    Belongs to the MGMT family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab185395 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185395 は論文での使用が確認できていません。

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